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Method for culturing hydrogen autotrophic denitrification granular sludge

A technology of autotrophic denitrification and nitrification granules, which is applied in the field of cultivating hydrogen autotrophic denitrification granular sludge, to achieve the effects of increasing sludge age, increasing operating load, and good settling performance

Active Publication Date: 2020-07-14
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there has not been any report on the cultivation method of hydroautotrophic denitrification granular sludge. Therefore, it is necessary to find a feasible cultivation method of hydroautotrophic denitrification granular sludge to promote the aggregation of hydroautotrophic denitrification microorganisms and the separation of particles. Formation, so that the cultivated particles have good sedimentation performance and denitrification activity, which has very important practical significance and economic value for further increasing the operating load of the hydrogen autotrophic denitrification process and promoting the development and application of the process

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  • Method for culturing hydrogen autotrophic denitrification granular sludge
  • Method for culturing hydrogen autotrophic denitrification granular sludge
  • Method for culturing hydrogen autotrophic denitrification granular sludge

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1, cultivating hydrogen autotrophic denitrification granular sludge

[0051] 1) Domestication and cultivation of hydroautotrophic denitrification flocculent sludge

[0052] Take the activated sludge of ordinary municipal sewage plant as inoculation sludge, the concentration is about 2.3gVSS / L, after inoculation, use H 2 Semi-continuous culture with self-made culture medium for 60 days. The composition of the culture solution is (contains the following substances per liter): 1.62gKNO 3 ,0.5gNaHCO 3 ,0.55gNaH 2 PO 4 2H 2 O, 2.11g K 2 HPO 4 ·3H 2 O and trace elements 7.3mgCaCl 2 2H 2 O,5.0mgFeSO 4 ·7H 2 O,2.5mgMnCl 2 4H 2 O,0.5mgCoCl 2 ·6H 2 O, 0.5mg (NH 4 ) 6 Mo 7 o 24 4H 2 O,0.22mgZnSO 4 ·7H 2 O and 0.2 mg CuSO 4 ·5H 2 O. The method of domestication and culture is sequential batch, and the sequence batch cycle is 12h. The pH of the culture system is 7.0±0.5, and the temperature is 30±1.0°C.

[0053] 2) The hydroautotrophic denitrifi...

Embodiment 2

[0057] Example 2, using hydrogen autotrophic denitrification granular sludge reactor to treat simulated groundwater

[0058] Utilize the hydroautotrophic denitrification granular sludge cultivated in Example 1 to treat groundwater, maintain the reaction temperature at 30±0.5°C, add an appropriate amount of KNO 3 The simulated groundwater was used as the reactor feed water, and the denitrification effect, stability and effluent NO of the reactor were investigated. 2 - -N level.

[0059] The sludge used in this test is the hydroautotrophic denitrification granular sludge cultivated in Example 1, and the inoculation sludge concentration in the reactor is 2.44g VSS / L.

[0060] The granular sludge reactor operates continuously for 12 days in accordance with the SBR method, and each SBR cycle is 35 minutes, including water inflow for 3 minutes, reaction for 22 minutes, precipitation for 8 minutes, and drainage for 2 minutes. The test water is to add a certain amount of KNO 3 tap...

Embodiment 3

[0064] Example 3, using hydrogen autotrophic denitrification granular sludge reactor to treat simulated groundwater under low temperature conditions

[0065] Utilize the hydroautotrophic denitrification granular sludge cultivated in Example 1 to treat groundwater, maintain the reaction temperature at 15±0.3°C, add an appropriate amount of KNO 3 The simulated groundwater was used as the reactor feed water, and the denitrification effect, stability and effluent NO of the reactor were investigated. 2 - -N level.

[0066] The sludge and reactor used in this test are the hydrogen autotrophic denitrification granular sludge and sludge after the end of Example 2.

[0067] The granular sludge reactor operates continuously for 11 days in accordance with the SBR method, in which the cycle is 85 min for 7 days and the cycle is 75 min for 4 days. When the cycle is 85 minutes, water is fed for 3 minutes, reaction is 72 minutes, precipitation is 8 minutes, and water is drained for 2 minu...

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Abstract

The invention discloses a method for culturing hydrogen autotrophic denitrification granular sludge. The method comprises the following steps: 1) domesticating and culturing hydrogen autotrophic denitrification flocculent sludge; 2) inoculating the flocculent sludge cultured in the step 1) into a reactor; (3) continuously operating for 8-15 days by adopting an SBR operation mode, wherein an operation period is 90-120 minutes; and taking simulated nitrate wastewater as inflow water; 4) continuously operating for 7-15 days by adopting an SBRoperation mode, wherein the operation period is 30-40minutes; and using the simulated nitrate wastewater as inflow water. The hydrogen autotrophic denitrification granular sludge prepared by the method is ochre, mature granules are basically ellipsoids,and the interior of each mature granule is relatively compact. The granular sludge has good settling performance, the microbial biomass in the reactor can be increased, the sludge age can be increased, and the operation load of a hydrogen autotrophic denitrification process can be increased.

Description

technical field [0001] The invention relates to a method for cultivating hydrogen autotrophic denitrification granular sludge. Background technique [0002] The hydrogen autotrophic denitrification process is a new type of autotrophic biological denitrification process. Its basic principle is to use hydrogen autotrophic denitrification bacteria to use hydrogen as the electron donor and nitrate or nitrite as the electron acceptor for respiration to remove nitrogen. Nitrate in water or wastewater, and the traditional heterotrophic biological denitrification process with organic substances as electron donors and the autotrophic biological denitrification process with other inorganic substances (such as elemental sulfur, sulfide, zero-valent iron, etc.) Compared with the nitrification process, the hydroautotrophic denitrification process has the advantages of clean product, no need for subsequent treatment, and no secondary pollution. It has a good application prospect in the tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F101/16
CPCC02F3/282C02F2101/163C02F2305/06
Inventor 左剑恶王雅娇王丝可
Owner TSINGHUA UNIV
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