Preparation method of electrochemical sensor for chloramphenicol content detection

Abstract

The invention relates to a preparation method of an electrochemical sensor for chloramphenicol content detection. The preparation method comprises the following steps: using a metal nitrate compound as a raw material to prepare a metal organic framework material A; synthesizing an organic framework material B by using a hydrothermal synthesis method; preparing a metal organic framework material B / nucleic acid aptamer signal probe chain by using a crosslinking method; and preparing a gold nanoparticle / functionalized metal organic framework material A gold electrode by using an electrochemical deposition method. In vitro binding arched DNA structures, the content of chloramphenicol in the sample is indicated by increasing a signal molecule response signal carried by a signal molecule labeledmetal organic framework material B / nucleic acid aptamer signal probe chain and the electrochemical sensor for chloramphenicol content detection is obtained; compared with other methods for chloramphenicol content detection, the prepared novel electrochemical sensor is high in response speed, high in signal-to-noise ratio, high in sensitivity, good in repeatability and high in accuracy.

Description

technical field [0001] The invention relates to a method for preparing an electrochemical sensor for detecting the content of chloramphenicol, in particular to a method for preparing a gold electrode based on gold nanoparticles / functionalized metal with a frame. Background technique [0002] With the continuous development of my country's economy, food safety has gradually become the focus of attention, and new food safety issues continue to emerge, which are related to the health, life safety and social economy of the people. One of the characteristics of my country's food safety is "serious pollution at the source". In order to prevent and treat livestock diseases, the animal husbandry industry abuses antibiotics as veterinary drugs, resulting in drug residues in animal foods, which will not only bring potential hidden dangers to the safety of animal foods, It will also cause harm to human health and the living environment. Among them, chloramphenicol, as the first class o...

AI Technical Summary

Problems solved by technology

In order to further improve the performance of sensors, nanomaterials are often used to construct biosensors as a signal amplification strategy to improve the sensitivity of sensors. Metal-organic frameworks (MOFs) are emerging nanomaterials in recent years. Due to their unique structural characteristics and excellent The electrical properties have been widely used in the field of electrochemical analysis, but the traditional 3D structure MOF has a large particle size, which makes it difficult to uniformly disperse it in aqueous solution, resulting in its instability and easy fall off when it is modified to the electrode surface , and there are disadvantages such as poor conductivity and low electrical activity, which limit its further application in electrochemical aptasensors. The design and construction of MOF materials combined with high-conductivity nanomaterials has become the key to the preparation of high-efficiency chloramphenicol electrochemical biosensors

At the same time, the traditional electrochemical aptamer detection strategy may have too little or no response when the target concentration is too low, making the signal difference before and after the detection too small, resulting in inaccurate detection results and unable to achieve ultra-sensitive detection. To address this shortcoming, an exonuclease-assisted circular amplification strategy provides an option for the realization of signal amplification

Currently, electrochemical aptasensors constructed using metal organic frameworks / highly conductive nanomaterials as electrode modification materials and exonuclease-assisted dual signal amplification strategies using metal organic frameworks loaded with signal molecules as signal tags have not yet used chlorine. In the detection application of mycin