Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of electrochemical aptamer ratio sensor for patulin detection

A patulin and electrochemical technology, applied in the field of electrochemical aptamer ratio sensor preparation, achieves the effects of low background noise, fast response speed and good repeatability

Active Publication Date: 2020-07-10
HENAN UNIVERSITY OF TECHNOLOGY
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the synthesis of functionalized trimetallic nanomaterials as electrode modification materials and the design and construction of ratiometric electrochemical aptasensors for the detection of patulin have not been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Specific steps are as follows:

[0025] (1) Tri-metallic composite nanoflowers were prepared by hydrothermal synthesis. Weighed 2 mg of tetrachloroauric acid, 10 mg of platinum diacetylacetonate, 10 mg of palladium chloride and 20 mg of nickel tetracarbonyl in 8 mL volume ratio of 1 : 4 in a mixed organic solvent of acetic acid and dimethylformamide, sonicated for 10 min, kept at 120°C for 1 h, collected the product by centrifugation, washed with methanol and cooled to 20°C to obtain a trimetallic composite nanoflower;

[0026] (2) Polish the gold electrode with alumina powder, place the polished gold electrode in ethanol and ultrapure water for 5 min, and then immerse the gold electrode in concentrated H with a volume ratio of 7:3. 2 SO 4 with 30%H 2 o 2 15 min in the strong oxidizing solution, rinsed and dried with distilled water, and put the gold electrode in the amino functionalized solution of 80 mM tetrabutylammonium tetrafluoroborate and p-nitrobenzene tetraf...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of an electrochemical aptamer ratio sensor for patulin detection. The preparation method comprises the following steps: preparing trimetal nanoflowers bya hydrothermal synthesis method to be used as a substrate material; modifying a substrate material and a nucleic acid aptamer on the surface of the gold electrode by covalent binding; and preparing anucleic acid single-stranded probe by adopting an enzyme circulation cutting method to prepare a nucleic acid aptamer / trimetal composite nanoflower / aminated gold electrode. The electrode system is adopted, the electrochemical aptamer sensor for detecting the content of patulin is obtained by taking the nucleic acid aptamer / trimetal composite nanoflower / amination gold electrode as the working electrode, the platinum wire electrode as the counter electrode and saturated silver chloride as the reference electrode and measuring the concentration of patulin through the change of the signal ratio ofthe electroactive substance. Compared with a common single-signal electrochemical sensor, the electrochemical aptamer ratio sensor has the advantages of being high in response speed, low in background noise, high in sensitivity, good in repeatability and high in accuracy.

Description

technical field [0001] The invention relates to a method for preparing an electrochemical aptamer ratio sensor for patulin detection, in particular to a method for preparing a gold electrode modified based on trimetallic nanometer materials. Background technique [0002] Food is the most basic material condition for the survival and development of human society. The state of food safety is directly related to the health and life safety of the broad masses of the people, and is related to the coordinated development of my country's national economy and society. At present, patulin contamination in food is a worldwide problem, especially in fruits and fruit products. In moldy apples, patulin contamination is particularly serious. Patulin is mainly derived from Penicillium, which can reproduce and produce toxins by using nutrients in fresh fruit pulp and other foods and feeds. In the processing of fruit products, long-term storage of raw materials often causes the content of p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 何保山路夏赵文红任文洁王金水惠明姜利英魏涛吴立根卫敏金华丽王涛
Owner HENAN UNIVERSITY OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com