Method for Isolating and Purifying Efficient Petroleum Hydrocarbon Degrading Bacteria from Crude Oil Contaminated Soil

Abstract

The invention provides a method for isolating and purifying high-efficiency petroleum hydrocarbon-degrading bacteria from crude oil-contaminated soil, which comprises mixing and culturing inorganic salt culture medium and high-oil-containing soil samples, and using thin oil / inorganic salt culture medium to culture the culture solution after mixed culture Carry out acclimatization culture, and then use thick oil / inorganic salt medium to carry out acclimatization culture on the culture solution after acclimatization culture, the obtained culture solution is diluted and spread on crude oil / inorganic salt solid plate medium and cultivated; and then purified to obtain For purified strains with consistent morphology, culture the purified strains in beef extract peptone medium, inoculate the resulting bacterial liquid into crude oil / inorganic salt liquid medium for crude oil degradation experiments; determine the crude oil degradation rate of the purified strains, and select the crude oil degradation rate Larger strains. The method can obtain petroleum hydrocarbon degrading bacteria capable of broad-spectrum degrading various components in crude oil, and has wide applicability and high use value.

Description

technical field [0001] The invention relates to a method for separating and purifying high-efficiency petroleum hydrocarbon degrading bacteria from crude oil polluted soil, and belongs to the technical field of petroleum hydrocarbon biodegradation treatment. Background technique [0002] As an important energy source, petroleum is the "blood" of modern industry, but while petroleum brings convenience to human beings, it also causes more and more serious environmental pollution. Due to accidents or improper management during oil extraction, transportation, storage, and processing, oil will be discharged into the soil, groundwater, ocean, etc., polluting the environment and directly endangering human production and life. There are mainly physical, chemical and microbiological methods for oil pollution control. Physical and chemical methods have the disadvantages of high energy consumption, high cost, and serious secondary pollution. Microbial methods can transform and remove ...

AI Technical Summary

Problems solved by technology

However, the strains screened by it have poor tolerance and adaptability to heavy components in crude oil, which inhibits the degradation of heavy oil with high content of heavy components. Therefore, the strains should be improved for various components The ability to adapt to different crude oil pollution environments and promote its degradation efficiency for different components of crude oil

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