Non-contact full-field deformation measurement method of amniotic membrane tissue using methylene blue for speckle making

Abstract

The invention provides a non-contact full-field deformation measurement method of an amniotic membrane tissue using methylene blue for speckle making, and belongs to the technical field of deformationmeasurement. The method comprises the steps of cutting an amniotic membrane sample, clamping the amniotic membrane sample on a stretcher, injecting a methylene blue solution into a fine particle sprayer, releasing the methylene blue solution in a space above the amniotic membrane by using the sprayer above the amniotic membrane sample, and enabling the methylene blue solution to freely fall ontothe surface of the amniotic membrane under the action of gravity; then checking speckle morphology, ensuring that speckles completely cover the surface to be measured, collecting deformation images byusing a high-resolution camera, and optimizing DIC calculation parameters by using a morphological method; and finally, conducting DIC calculation to obtain a full-field deformation result of the biological membrane tissue. The method is suitable for manufacturing speckle patterns of biological film tissues such as amniotic membrane biological tissues, has the characteristics of good speckle adhesiveness, no toxicity or harm, rapidness and simplicity in implementation and easiness in operation, and is low in cost and convenient for large-scale popularization and use.

Description

technical field [0001] The invention relates to the technical field of deformation measurement, in particular to a non-contact full-field deformation measurement method of amniotic membrane tissue using methylene blue plaque making. Background technique [0002] The amniotic membrane is a layer of biofilm that wraps the embryo and amniotic fluid during pregnancy. It mainly protects the embryo and provides a stable pregnancy environment. Studying the mechanical properties of amniotic membrane will not only help in the research of pregnancy assistance, but also help other medical researches that use amniotic membrane as a biological scaffold material. In order to study the full-field force deformation of amniotic membrane, it is necessary to measure the deformation of amniotic membrane under uniaxial tensile test with the help of DIC method. [0003] Digital Image Correlation (DIC) was first proposed independently in the early 1980s by Ichiro Yamaguchi of Japan and Peter and ...

AI Technical Summary

Problems solved by technology

However, for analytes such as biofilm tissue, such as human amniotic membrane tissue, there are the following difficulties in using traditional spotting methods:

[0005] 1) The color of the amnion tissue is translucent, and there is no natural texture on the surface, so the surface texture cannot be directly used as speckle for measurement, and the speckle pattern can only be artificially prepared on the surface

[0006] 2) There is a large amount of water attached to the surface of the amnion and the surface is extremely smooth, which makes it difficult for traditional oil-based paints to adsorb on the surface, and it is prone to problems such as speckle and biofilm surface are not firmly bonded, slipping or peeling off during stretching

However, due to the light texture of amnion itself, this powder coating will introduce too much weight, which will affect the mechanical properties of amnion tissue during the experiment.

[0008] 4) The diluents used in most of the existing self-spray paints have certain toxicity and are harmful to the human body

Said method mainly has the following problems: 1, the used diluent of oil-based paint has certain toxicity, which is harmful to human body

2. Since the surface of the amniotic membrane is moist and smooth, the adhesion of oil-based paint to the surface of the amniotic membrane is poor

3. The reliability of oil-based paint is poor in the case of large deformation

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