Factor slow-release neutral gel system for 3D printing or in-situ injection and preparation method of factor slow-release neutral gel system
A technology of in situ injection and 3D printing, which is applied in the fields of pharmaceutical formula, medical science, and prosthesis, and can solve the problems of losing gel performance, limiting operation time, and affecting collagen, etc.
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Embodiment 1
[0032] 1. Collagen extraction:
[0033] Bovine Achilles tendon was crushed and degreased, immersed in 0.1mol / L acetic acid solution, and pepsin (3mg / mL) was added. After stirring at 4°C for 72 hours, the 4mol / L sodium chloride solution was salted out, and the resulting precipitate was dialyzed with 0.1mol / L acetic acid, and the molecular weight cut-off of the dialysis bag was 8000Da. Use acetic acid solution to adjust the concentration to obtain 6 mg / mL bovine Achilles tendon collagen, and the solvent acetic acid concentration is 0.1 mol / L. The above steps are all aseptic.
[0034] 2. Preparation of collagen fibers:
[0035] Mix 8.09 mL of the above collagen solution, 1 mL of 10-fold concentration of PBS, 0.81 mL of 1 mol / L sodium hydroxide solution and 0.1 mL of 1 mg / mL phenol red to obtain a red neutral collagen solution. The solution was added into 10 times the volume of 0.01 mol / L PBS, stirred magnetically at 37° C., and the stirring speed was 1000 rpm. After stirring f...
Embodiment 2
[0044] 1. Collagen extraction:
[0045] Bovine Achilles tendon was crushed and degreased, immersed in 0.1mol / L acetic acid solution, and pepsin (3mg / mL) was added. After stirring at 4°C for 72 hours, the 4mol / L sodium chloride solution was salted out, and the resulting precipitate was dialyzed with 0.1mol / L acetic acid, and the molecular weight cut-off of the dialysis bag was 8000Da. Use 0.1 mol / L acetic acid solution to adjust the concentration to obtain 6 mg / mL bovine Achilles tendon collagen, and the above steps are all aseptic operations.
[0046] 2. Preparation of collagen fibers:
[0047] Mix 8.09 mL of the above collagen solution, 1 mL of 10-fold concentration PBS, 0.81 mL of 1 mol / L sodium hydroxide and 0.1 mL of 1 mg / mL phenol red to obtain a red neutral collagen solution. . The solution was added into 10 times the volume of 0.01 mol / L PBS, stirred magnetically at 25° C., and the stirring temperature speed was 2000 rpm. After 1 hour, centrifuge at 10,000 rpm, pour...
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