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Method for producing coumestrol by using cultured roots of leguminous plant

A technology of coumestrol and production method, which is applied in the directions of biochemical equipment and methods, tissue culture, cell culture medium, etc., can solve the problems of short-term, small amount of coumestrol, difficulty in commercialization and the like, and achieve cost saving , the effect of saving time

Pending Publication Date: 2020-06-26
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, since the amount of coumestrol present in some plants is very small, the price of natural coumestrol currently commercially available is very expensive
Therefore, there was a brief attempt to obtain coumestrol by synthesis, however, due to the complex chemical structure (fused with a large number of aromatic rings), a route to the development of a simple synthesis of coumestrol has not yet been found
Although several synthetic methods have been reported, each method has various problems as explained below, thereby presenting difficulties in commercialization

Method used

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  • Method for producing coumestrol by using cultured roots of leguminous plant
  • Method for producing coumestrol by using cultured roots of leguminous plant
  • Method for producing coumestrol by using cultured roots of leguminous plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] [Example 1] Germination of bean seeds and induction of isolated plants

[0062] After sterilizing each surface of bean seeds (Glycine max, preferably mythical bean variety) and Bean bean with a 2% by weight aqueous solution of sodium hypochlorite (Sodium Hypochlorite) for 20 minutes, they were washed 3 times with sterile water. Then, according to the bean species, 0.5-1.0MS medium (Murashige and Skoog Medium, Haarlem, Netherlands) supplemented with 30g / L sucrose was used to induce the germination of plants in the reactor under the light condition of 25±1°C for 2 weeks. .

Embodiment 2

[0063] [Example 2] Induction and Proliferation of Bean Cultured Roots

[0064] After the radicle induction culture root of the plant body germinated from the reactor, in the spherical (BulbType) bioreactor (generally can buy from the market, refer to that the air volume is 3L) figure 1 ) within 2L of 0.5MS medium ( Murashige and Skoog medium, Duchefa, Netherlands) proliferated root cultures for 4 weeks. In addition, the roots were multiplied and cultured for 4 weeks under the same conditions as above using 1.0 MS medium. The 0.5MS medium is a medium prepared by setting the concentration of raw materials such as inorganic substances used in the medium to 1 / 2 of that of a normal MS medium. same base medium. The medium was sterilized at 121°C and 1.2 atmosphere for 35 minutes after adjusting the pH to 5.8 with 1N NaOH. The cultured roots were cut into 1-1.5 cm and inoculated into the culture medium at an inoculation density of 4 g / L on the basis of fresh weight, and then cult...

Embodiment 3

[0066] [Example 3] Preparation of extract and enzyme treatment

[0067] 1. Preparation of Extract

[0068] The cultured roots of beans harvested and dried according to the above Examples 1 and 2 were dipped into 80% (w / v) ethanol water so that the weight ratio of cultured roots: ethanol water was 1:30 to 1:50 (preferably 1:30 ), and extracted at room temperature for 24 hours. The extract was filtered through a filter paper, and the solvent was evaporated to dryness to obtain a powder (extract). In addition, through repeated extraction experiments, it can be confirmed that when using the cultured root produced in Example 1 to prepare the extract, the components in the extract remain uniform, and it is confirmed that a large amount of extraction can be carried out by combining the extract preparation methods such as subculture. Production.

[0069] 2. Enzyme treatment

[0070] After the extract powder was treated with the same weight of liquid-phase pectinase (pectinase) enz...

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PUM

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Abstract

The specification relates to a method for producing coumestrol by using cultured roots of leguminous plants and a method for preparing a leguminous plant extract having an increased coumestrol amount.A method according to one aspect of the present invention enables a large amount of cumestrol-to-be-produced to be uniformly obtained by using leguminous plants and enzymes so as to save more costs and time than a conventional method, and can be safely used in various fields, such as in pharmaceuticals, foods or cosmetics since cumestrol is produced using a natural material.

Description

technical field [0001] The invention relates to a method for producing coumestrol by using cultured roots of leguminous plants and a method for preparing coumestrol with increased content of coumestrol. Background technique [0002] Since the US FDA banned the use of inexpensive synthetic estrogens with potent estrogenic effects, such as diethylstilbestrol (DES), in estrogen therapy for menopausal women, natural phytoestrogens that can replace estrogen have attracted research interest . However, since these substances exist in nature in extremely small amounts, it is difficult to obtain them in large quantities. Additionally, more than half a century has passed without significant progress due to a lukewarm attitude towards the large-scale synthesis of these substances. Although the development of synthetic methods has been intermittently reported, they are only on a laboratory scale, and there is currently no easy method for large-scale production. [0003] So far, coume...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12N5/00C12N9/24
CPCC12N5/00C12N9/24C12P17/18C12P17/181C12N5/0025C12N9/2402C12Y302/01015
Inventor 李恩姃姜永圭朴俊星
Owner AMOREPACIFIC CORP
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