Bionic heterogeneous integrated bone-cartilage repair stent and preparation method thereof

A technology for cartilage repair and calcification of cartilage, applied in prosthetics, tissue regeneration, medical science, etc., can solve the problems of no multi-level structure, weak interface binding force, etc., and achieve good antibacterial properties, low cost, and strong plasticity

Active Publication Date: 2020-06-26
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the invention patent with the application number of 201810059754.2 discloses a biomimetic oriented osteochondral scaffold released by two-factor programming. The upper surface layer and the lower transitional layer prepared separately are bonded by silk fibroin to for...

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  • Bionic heterogeneous integrated bone-cartilage repair stent and preparation method thereof

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Embodiment 1

[0053] 1. Preparation of Subchondral Bone Layer Precursor

[0054] (1) Dissolving 1.00g chitosan in an acetic acid solution with a volume fraction of 2%, magnetic stirring for 30min under 37°C water bath conditions, to obtain chitosan acid solution;

[0055] (2) Add 8 mL each of 2 mol / L calcium nitrate solution and 1.2 mol / L dipotassium hydrogen phosphate solution to the mixed solution obtained in step (1) in turn, stir well to make it evenly mixed, and obtain the subchondral bone layer complex Precursor solution; wherein the Ca / P molar ratio is n(Ca 2+ ):n(PO 4 3- )=1.67:1;

[0056] (3) Inject the chitosan prepared in (2) and the uniform mixture of inorganic ions into the mould, and freeze in a refrigerator at -20°C to form solid A;

[0057] 2. Preparation of precursor composites of calcified cartilage layer and subchondral bone layer

[0058] (4) dissolving 0.75g chitosan in an acetic acid solution with a volume fraction of 2%, magnetic stirring for 30min under 37°C wat...

Embodiment 2

[0071] 1. Preparation of Subchondral Bone Layer Precursor

[0072] (1) Dissolving 1.00g chitosan in an acetic acid solution with a volume fraction of 2%, magnetic stirring for 30min under 37°C water bath conditions, to obtain chitosan acid solution;

[0073] (2) Add 8 mL each of 2 mol / L calcium nitrate solution and 1.2 mol / L dipotassium hydrogen phosphate solution to the mixed solution obtained in step (1) in turn, stir well to make it evenly mixed, and obtain the subchondral bone layer complex Precursor solution; wherein the Ca / P molar ratio is n(Ca 2+ ):n(PO 4 3- )=1.67:1;

[0074] (3) Inject the chitosan prepared in (2) and the uniform mixture of inorganic ions into the mould, and freeze in a refrigerator at -20°C to form solid A;

[0075] 2. Preparation of precursor composites of calcified cartilage layer and subchondral bone layer

[0076] (4) dissolving 0.75g chitosan in an acetic acid solution with a volume fraction of 2%, magnetic stirring for 30min under 37°C wat...

Embodiment 3

[0089] 1. Preparation of Subchondral Bone Layer Precursor

[0090] (1) Dissolving 1.25g chitosan in an acetic acid solution with a volume fraction of 2%, magnetic stirring for 30min under 37°C water bath conditions, to obtain chitosan acid solution;

[0091] (2) Add 8 mL each of 2 mol / L calcium nitrate solution and 1.2 mol / L dipotassium hydrogen phosphate solution to the mixed solution obtained in step (1) in turn, stir well to make it evenly mixed, and obtain the subchondral bone layer complex Precursor solution; wherein the Ca / P molar ratio is n(Ca 2+ ):n(PO 4 3- )=1.67:1;

[0092] (3) Inject the chitosan prepared in (2) and the uniform mixture of inorganic ions into the mould, and freeze in a refrigerator at -20°C to form solid A;

[0093] 2. Preparation of precursor composites of calcified cartilage layer and subchondral bone layer

[0094] (4) dissolving 1.00 g of chitosan in an acetic acid solution with a volume fraction of 2%, and magnetic stirring for 30 min under...

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Abstract

The invention provides a bionic heterogeneous integrated bone-cartilage repair stent and a preparation method thereof. The repair stent is made of a multilayered structure stent material and sequentially comprises a hyaline cartilage layer, a calcified cartilage layer and a subchondral bone layer from top to bottom, wherein the hyaline cartilage layer is of an interpenetrating porous structure formed by interweaving two biocompatible materials including hyaluronic acid and chitosan; the calcified cartilage layer is of an oriented gradient porous structure formed by compositing chitosan and nano-hydroxyapatite; and the subchondral bone layer is of a homogeneous porous structure formed by hybridizing bone matrix component like chitosan and nano-hydroxyapatite. An in-situ synthesis method isadopted and combined with a directed freezing technology to construct an integrated multilayer repair stent material with a bone-like-cartilage heterostructure. The composite material synthesized by the preparation method combines advantages of all components, and has the effects of good antibacterial activity, biocompatibility and in-situ induction of two-way regeneration of bone and cartilage; and the binding force of an interface is enhanced, so that the structure integration of the stent is realized.

Description

technical field [0001] The invention belongs to the field of composite materials, and in particular relates to a bionic heterogeneous integrated bone-cartilage repair bracket and a preparation method thereof. Background technique [0002] Articular cartilage damage caused by trauma, disease, degeneration and other factors is a common orthopedic disease in clinical practice. Especially in the face of the aging trend of the global population, the tissue damage of the articular cartilage is particularly prominent, and the expensive medical expenses are a burden to the patient's family, The society and the country have brought huge mental pressure and heavy economic burden. Because articular chondrocytes are cells in the terminal stage of differentiation, and there are no blood vessels, lymphatic vessels, and nerves in the articular cartilage, and lack of corresponding nerve and humoral regulation, the self-repair ability of cartilage defects is limited. Once damaged, the subcho...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/56A61L27/46A61L27/48A61L27/54
CPCA61L27/46A61L27/48A61L27/54A61L27/56A61L2300/404A61L2430/02A61L2430/06
Inventor 李建树赵耀陈卓鑫丁春梅
Owner SICHUAN UNIV
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