Simultaneous detection method for illegally added medicines in tranquilizing and allaying excitement health-care product

Abstract

The invention, which relates to the field of health care product detection, provides a simultaneous detection method for illegally added medicines in a tranquilizing and allaying excitement health-care product. Specifically, the method for simultaneous determination of illegally added drugs such as benzodiazepines, barbital and atypical benzodiazepines hypnotics in tranquilizing and allaying excitement health-care products by ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS-MS) comprises the steps of extraction, purification, membrane filtration, tandem triple quadrupolemass spectrometry detection and establishment of a standard curve. The method is simple in steps, high in accuracy and good in reproducibility, can be used for qualitatively and quantitatively analyzing various illegally added medicines in the health care product at the same time, and has the advantage of rapid and high-throughput detection.

Description

technical field [0001] The invention belongs to the field of detection of health care products and provides a simultaneous detection method for illegally added drugs in health care products for tranquilizing and calming the nerves. Background technique [0002] Sleep is an important and necessary physiological need of the human body. Yet along with the quickening of social life rhythm, irregular, the increasing of pressure day by day, insomnia phenomenon becomes more and more serious. Modern medicine has carried out in-depth research on insomnia, and has gradually found a series of drugs for the treatment of insomnia, mainly containing barbiturates (first class), benzodiazepines (second class) and atypical benzodiazepines. These drugs have strong sedative and hypnotic effects, but long-term use can produce dependence and even toxic side effects. Therefore, some health care products with traditional Chinese medicines and proprietary Chinese medicines as the main ingredients...

AI Technical Summary

Problems solved by technology

Among them, the detection resolution of thin-layer chromatography and high-performance liquid chromatography is weak, and false positives often occur due to the many impurities in health food; the sensitivity of gas chromatography-mass spectrometry is poor; because liquid chromatography-tandem mass spectrometry has high sensitivity, The advantage of strong anti-interference ability is the most effective method to identify illegally added chemical drugs in health products

[0004] The State Food and Drug Administration's "Supplementary Inspection Methods and Inspection Items for Drug Inspection Approval No. 2009024" adopts thin-layer chromatography for initial screening, liquid chromatography for qualitative, positive sample liquid chromatography tandem mass spectrometry full-scan confirmation method is cumbersome, and literature search The items detected by liquid chromatography tandem mass spectrometry are relatively single, and the coverage is insufficient

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