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Method for controlling growth and development of plant roots by mutually combining MYB and UVR8 in UV-B dependent form

A UV-B, plant root technology, applied in the field of biotechnology and botany

Active Publication Date: 2020-06-19
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, most of the output phenotypes of the above studies are the regulation of hypocotyls and stress regulation. Does UV-B participate in the regulation of other plant organs such as root development? Are there transcription factors that directly bind UVR8 in a UV-B-dependent form? Currently UVR8 is the only known UV-B receptor, and the only protein that interacts with UVR8 in a UV-B-dependent manner is COP1. More proteins that interact with UVR8 in a UV-B-dependent manner need to be discovered for optogenetics study

Method used

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  • Method for controlling growth and development of plant roots by mutually combining MYB and UVR8 in UV-B dependent form
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  • Method for controlling growth and development of plant roots by mutually combining MYB and UVR8 in UV-B dependent form

Examples

Experimental program
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Embodiment 1

[0134] Example 1, UVR8 is involved in regulating the lateral root growth of Arabidopsis under UV-B

[0135] In order to study the plant physiological phenotypes regulated by UV-B, the inventors planted Arabidopsis wild-type (WT) vertically on 1 / 2 MS plates, and placed them under continuous white light and white light plus ultraviolet light conditions for 2 weeks. Observe physiological phenotypes under white light and white light plus UV.

[0136] The inventors found that UV-B can induce photomorphogenesis and anthocyanin accumulation phenotypes as well as root phenotypes, UV-B can inhibit the growth of WT roots, and the growth of seedlings under UV-B and white light Compared with seedlings, the elongation of main root and lateral root was significantly inhibited ( figure 1 A). UV-B inhibition of lateral root growth is dependent on its receptor UVR8, because the lateral root length of the uvr8 mutant (T-DNA insertion mutant SALK_033468) grown under UV-B is longer than that of...

Embodiment 2

[0139] Example 2. Monomer UVR8 inhibits auxin response and is rooted in the main reaction

[0140] UVR8 responds to UV-B in two ways, one is that UVR8 will be enriched in the nucleus after UV-B irradiation, and the other is that UVR8 will dissociate from homodimers to form monomers after UV-B irradiation. In order to further determine which response of UVR8 is crucial for the growth and development of lateral roots, the present inventors expressed WT, persistently activated (monomeric form) UVR8 (GR- UVR8 R338A / uvr8) and expression of nuclear localization-induced persistently inactive (dimeric form) UVR8 (GR-UVR8 W285F / uvr8) were planted on 1 / 2MS plate, placed in long-day light for 5 days, then moved to 1 / 2MS plate added with 1μMIAA, and placed in white light plus UV for 1 week. It was found that GR-UVR8 R338A / uvr8 and GR-UVR8 W285F / uvr8 showed the phenotype of uvr8 mutant, that is, high concentration of auxin inhibited the growth of lateral roots, indicating that the n...

Embodiment 3

[0142] Example 3, UV-B inhibits the expression of auxin response genes through UVR8

[0143] UV-B regulates lateral root growth in a UVR8-dependent manner, and UVR8 can inhibit auxin responses, but how UVR8 affects auxin signaling remains to be further studied.

[0144] First, the inventors analyzed published transcriptome data, including genes regulated by UV-B and genes affected by auxin in WT and uvr8 mutants (ArrayExpress, E-MEXP-1957, E-GEOD-627 ). The results of the analysis found that 67% of the genes affected by UV-B and auxin changed in the opposite direction and most of them depended on UVR8 ( Figure 4 A). The present inventors further analyzed the genes involved in auxin response separately, and found that the expression of these genes was induced by auxin, but was inhibited by UV-B in a UVR8-dependent manner, and the inhibitory effect of UV-B basically disappeared in the uvr8 mutant ( Figure 4 B). The present inventors detected the expression of genes related...

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Abstract

The invention relates to a method for controlling the growth and development of plant roots by mutually combining MYB and UVR8 in a UV-B dependent form and an application based the method. The invention discloses a method for controlling the growth and development of the plant roots and particularly lateral roots by controlling, by UV-B, photomorphogenesis and stress response by virtue of UVR8 andinhibiting an auxin signal by virtue of UVR8. Meanwhile, the invention further discloses two transcription factors MYB73 and MYB77 interacting with UVR8 in the UV-B dependent form, UV-B induces UVR8to interact with MYB73 / MYB77 to inhibit the DNA binding activity of MYB73 / MYB77, and thus, the auxin signal is controlled to control the growth of the plant roots and particularly the lateral roots. The technical solution provided by the invention is not only helpful to study how plants adapt to the environment better, but also provides a novel tool for photogenetics.

Description

technical field [0001] The present invention belongs to the fields of biotechnology and botany; more specifically, the present invention relates to the mutual combination of MYB73 / MYB77 and UVR8 in a UV-B-dependent manner to regulate the growth and development of plant roots, and applications thereof. Background technique [0002] Ultraviolet light can be divided into UV-A, UV-B and UV-C according to different wavelengths, among which UV-A and part of UV-B can reach the surface of the earth and regulate the growth and development of plants. UV-A is sensed by blue light receptors to regulate plant growth and development. The impact of UV-B on plant growth and development mainly includes two types: one is to regulate the photomorphogenesis of plants as a light signal, including inhibiting hypocotyl elongation, promoting cotyledon opening and deetiolation, etc.; the other regulation is Long-term or high-intensity UV-B radiation can cause damage to plant growth. However, there...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12Q1/6895A01H5/06A01H6/20A01H6/46A01H6/60A01H6/82
CPCC07K14/415C12N15/8261C12N15/8216C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 刘宏涛杨郁
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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