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Photoacoustic imaging contrast agent for cancer diagnosis as well as preparation method and application thereof

A cancer diagnosis and photoacoustic imaging technology, applied in the fields of nanotechnology and cancer diagnosis, to achieve the effect of reducing two-dimensional size, low density, and excellent real-time performance

Inactive Publication Date: 2020-06-16
张峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, although MXene materials have been paid attention to and have been improved in multiple directions, their application fields are mostly battery technology and membrane processing technology, and there are few photoacoustic imaging comparisons using MXene materials for cancer diagnosis. agent reports

Method used

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  • Photoacoustic imaging contrast agent for cancer diagnosis as well as preparation method and application thereof
  • Photoacoustic imaging contrast agent for cancer diagnosis as well as preparation method and application thereof
  • Photoacoustic imaging contrast agent for cancer diagnosis as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A method for preparing a photoacoustic imaging contrast agent for cancer diagnosis, comprising the steps of:

[0040] S1, in 1g Ti 3 AlC 2 Add 1 g of LiF solid, slowly add 10 mL of concentrated hydrochloric acid, stir at room temperature for 72 h, centrifuge at 8000 rpm to discard the supernatant, wash the product by centrifugation with deionized water, add 1 mL of DMSO as an intercalation agent, and sonicate for 1 hour to increase Ti 3 C 2 T x Layer spacing, centrifuged at 8000rpn, discarded supernatant, the product obtained was washed by centrifugation with deionized water, the centrifugal speed was 8000rpn, and the number of washings was 3 times. 3 C 2 T x powder;

[0041] S2, get 1g of Ti prepared in step S1 3 C 2 T x Ultrasonically disperse the powder in 50mL of ultrapure water, add 2g of dopamine hydrochloride, and stir at room temperature for 1h in the dark, then add 25mL of 0.1mol / L Tris buffer solution, add dilute hydrochloric acid dropwise to adjust ...

Embodiment 2

[0050] This example provides a method for preparing a photoacoustic imaging contrast agent for cancer diagnosis. Compared with Example 1, the difference is that the polydopamine modification in step S2 is not performed, and the rest are the same as in the example. Here No longer.

Embodiment 3

[0052] This example provides a method for preparing a photoacoustic imaging contrast agent for cancer diagnosis. Compared with Example 1, the difference is that the RGD polypeptide in step S6 is not covalently bound, and the rest are the same as in the example. I won't repeat them here.

[0053] Such as Figure 5As shown, in terms of in vivo experiments, the good tumor targeting effect was first verified by SMMC-7721 subcutaneous tumor-bearing mice, and the comparison of tumor targeting efficiency 4 hours after injection: Example 1 Targeting group: Example 2 Targeting group: Implementation Example 3 Targeting group = 17.85%: 11.67%: 12.05%, confirming that the present invention firstly synthesizes two-dimensional Ti modified by polydopamine whose surface is rich in -OH functional groups by chemical oxidation polymerization 3 C 2 T x Nanosheets, and through electrostatic self-assembly, the hollow ZnSn(OH) surface is positively charged after amination modification 6 Microsph...

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Abstract

The invention discloses a photoacoustic imaging contrast agent for cancer diagnosis as well as a preparation method and an application of the photoacoustic imaging contrast agent. The preparation method comprises the following steps: firstly, synthesizing a polydopamine-modified two-dimensional Ti3C2Tx nanosheet with the surface rich in -OH functional groups through a chemical oxidative polymerization method; coating the Ti3C2Tx nanosheet with hollow ZnSn(OH) 6 microspheres with positive electricity on the surfaces after amination modification through an electrostatic self-assembly effect, sothat a ZnSn (OH) 6 / Ti3C2Tx heterostructure is formed; combining RGD to the amination modified Ti3C2TxMXene nanosheet wrapped by the hollow ZnSn (OH) 6 through the covalent bonding effect, and obtaining the hollow ZnSn (OH) 6-coated Ti3C2TxMXene nanosheet. Therefore, the contrast agent provided by the invention has excellent tumor targeting to a tumor site, has excellent real-time performance, sensitivity and accuracy during tumor imaging, and can be used for tumor diagnosis, imaging technology-oriented treatment and real-time monitoring. The contrast agent disclosed by the invention still hasan excellent imaging effect when the intravenous injection dosage of the contrast agent is as low as 13.1 [mu]g / mL.

Description

technical field [0001] The invention relates to the technical fields of nanotechnology and cancer diagnosis, in particular to a photoacoustic imaging contrast agent for cancer diagnosis and its preparation method and application. Background technique [0002] In recent years, layered two-dimensional materials have been widely and deeply studied due to their unique properties, among which graphene nanosheets and black phosphorus nanosheets are the most studied. MXene is a new type of transition metal carbide or nitride material with a two-dimensional layered structure developed by Yury Gogotsi and Michel W. Barsoum of Drexel University in 2011, which has many properties similar to graphene , such as good electrical conductivity, larger specific surface area and higher strength. At present, about 70 kinds of MXene materials have been discovered, including Ti 3 C 2 、Ti 2 C, V 2 C. Nb 2 C. Nb 4 C 3 、 Ta 4 C 3 and Ti 4 N 3 Wait. Over the years, those skilled in the a...

Claims

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Application Information

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IPC IPC(8): A61K49/22
CPCA61K49/22
Inventor 张峰
Owner 张峰
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