Preparation method and application of rat chronic dorsal root neurothlipsis model

A technology of dorsal root nerve and model, applied in the field of pathology and zoology, can solve the problems of severe animal damage and difficult operation, and achieve the effect of small animal damage, good stability, and easy objective measurement.

Inactive Publication Date: 2020-06-16
嘉升(上海)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing models have obvious deficiencies in simulating lumbar disc herniation, or there is a certain gap with the actual clinical lesion, such as the chronic sciatic nerve ligation model; or the operation is difficult and causes serious damage to animals, such as the autologous nucleus pulposus transplantation model, selective Nerve root ligation model

Method used

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  • Preparation method and application of rat chronic dorsal root neurothlipsis model
  • Preparation method and application of rat chronic dorsal root neurothlipsis model
  • Preparation method and application of rat chronic dorsal root neurothlipsis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Preparation of model

[0034] 1. Rats (220-250g) were anesthetized by intraperitoneal injection of 1% sodium pentobarbital (40mg / kg), the back was shaved, the back skin was disinfected with iodophor, fixed on the animal operating table in the prone position and covered with sterile towels.

[0035] 2. Make a 2-3cm longitudinal incision on the left side of the rat's lumbar 4 to sacral 1 level, cut the skin, and bluntly separate the paraspinal muscles to the top of the transverse process, exposing the lumbar 4 intervertebral foramen and lumbar 5 intervertebral foramen ( The paraspinal muscles can be kept separated by self-made hooks).

[0036] 3. Insert two L-shaped rods 10 with the first end 11 of 4mm, the second end 12 of 2mm and the diameter of 0.6mm respectively into the lumbar 4 intervertebral foramen and lumbar 5 intervertebral foramen at a certain angle. The two ends 12 are placed outside the lumbar 4 intervertebral foramen and lumbar 5 intervertebral foramen....

Embodiment 2

[0040] Example 2. Effect detection of the model

[0041] The present invention has verified the effect of the model through experiments. Through 28 days of observation, it was determined that the model obtained in Example 1 stably and accurately simulated the claudication and pain symptoms after the lumbar disc herniated and compressed the nerve root. image 3 It shows that the postoperative rat's hind paw on the operating side (left side) (see part A) did not grasp the net frame with the other three paws, but curled up, showing the performance of not daring to bear weight.

[0042] Table 1 Rat foot withdrawal threshold on different days after operation (unit: g)

[0043]

[0044] Table 1 shows the specific mean value and standard error of the withdrawal threshold of both lower limbs of 11 rats within 28 days after the operation. It can be seen that the withdrawal threshold of the hind paw on the surgical side was significantly lower than that before and on the contralateral hind paw...

Embodiment 3

[0046] Example 3: Application of the model

[0047] The two groups of rats were modeled using the method described in Example 1. The material of the L-shaped rods was H62 brass or polylactic acid, which is not interfered by magnetic fields, and will not cause chemical stimulation to nerves when placed in the body. A material with a certain degree of plasticity. On the seventh day after modeling, rats were treated with repeated transcranial magnetic stimulation (rTMS). One group received real magnetic stimulation, which was the magnetic stimulation group; the other group received fake magnetic stimulation, which was the fake stimulation group. The results showed that the withdrawal threshold of rats in the magnetic stimulation group was significantly higher than that in the sham stimulation group. Figure 5 Shown. This indicates that magnetic stimulation relieves the pain caused by nerve compression, and this model can be used for research on magnetic stimulation therapy.

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Abstract

The invention discloses a preparation method and application of a rat chronic dorsal root neurothlipsis model. The method includes the steps: step one, narcotizing a rat; step two, cutting a longitudinal incision on the horizontal left or horizontal right of parts from the 4th lumbar vertebra to the 1st sacrrum, cutting the skin, performing blunt dissection on paraspinal muscles, and exposing the4th lumbar intervertebral foramina and the 5th lumbar intervertebral foramina; and step three, inserting compression elements into the 4th lumbar intervertebral foramen and the 5th lumbar intervertebral foramen on the left or the right so as to obtain the rat chronic dorsal root neurothlipsis model. The compression elements are inserted into the lumbar intervertebral foramina so that continuous and chronic mechanical compression symptoms, caused by protrusions of lumbar disc herniation, of nerve roots are simulated, and the etiology of the symptoms is close to clinical situations; and the preparation method is simple, and has small damage to the animal, good stability and a high success rate. The model which is obtained by using the method can be applied to research of treatment methods and / or imaging detection of lumbar disc herniation.

Description

Technical field [0001] The invention relates to the fields of zoology and pathology, in particular to a preparation method and application of a rat chronic dorsal root nerve compression model. Background technique [0002] Lumbar disc herniation is the main disease that causes work loss and activity restriction in most parts of the world. With the continuous acceleration of the rhythm of daily life, lumbar disc herniation has become a common and frequently-occurring disease in clinical spine surgery, and the number of patients is increasing. Shows the trend of younger and universal. The disease is mainly due to the long-term uneven force of the lumbar spine or sudden trauma, which leads to the deformation and rupture of the lumbar spine fibrous annulus, and the fibrous annulus, nucleus pulposus and cartilage endplate protrude independently or simultaneously, thereby compressing the sciatic nerve, nerve root or cauda equina Nerve, the patient has waist pain, radiating pain in the...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02A01K2227/105A01K2267/0356
Inventor 张也许东升赵丹周少婷
Owner 嘉升(上海)生物科技有限公司
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