Anti-echinococcosis antigen monoclonal antibody hybridoma cell strain, anti-echinococcosis antigen monoclonal antibody and application thereof
A hybridoma cell line and monoclonal antibody technology, applied in the fields of hybrid cell preparation, application, botanical equipment and methods, etc., can solve the problems of no simple and effective diagnostic method for human and canine early diagnosis, and the diagnosis method needs to be broken through.
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[0034] Embodiment: Before producing the monoclonal antibody and the hybridoma cell line secreting the monoclonal antibody, the following preparations need to be made:
[0035] 1. Preparation of experimental reagents: Triton X-100, bovine serum albumin (BSA), protein assayconcertration, protein marker, polyethylene glycol (PEG1500), RPMI-1640, fetal bovine serum purchased from Hyclone Company, 100×HT, 100×HAT was purchased from Hyclone Company, other reagents were of domestic analytical grade, low molecular mass standard protein marker was purchased from Promega Company; Tween-20, ammonium persulfate (APS), tetramethyldiethylamine (TEMED), 20× PBS concentrate bovine serum albumin (BSA), o-phenylenediamine (OPD), horseradish peroxidase-labeled goat anti-mouse IgG (HRP-IgG), dimethyl sulfoxide (DMSO), ELISA coating solution (pH7 .4), 8-azaguanine, and serum diluents are self-made by the laboratory; the rest of the reagents are domestic analytically pure;
[0036] 2. Preparation ...
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