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Brevibacillus laterosporus strain S62-9 and application thereof

A technology of Brevibacterium and strains, applied in the field of microorganisms, can solve the problems of narrow antibacterial spectrum, limited application of Brevibacterium lateralis, etc., and achieve the effects of high bacteriostasis, enhanced immunity, and improved utilization.

Active Publication Date: 2020-06-12
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as previously found, the strains of Brevibacillus lateralsporosa have a narrow antibacterial spectrum and only have certain antibacterial effects on certain types of pathogenic bacteria, such as only on common animal pathogenic bacteria Staphylococcus aureus, Streptococcus suis, Escherichia coli, One or several of Salmonella and Y. enterocolitica have a certain antibacterial effect, or have a certain antibacterial effect on the pathogenic fungi Fusarium moniliforme, Fusarium solani, and Asparagus stem blight One or more of cucumber, cucumber anthracnose and peach rhizopus have antagonistic effect
Due to the narrow inhibitory spectrum of the strains of Brevibacillus spp. so far, the application of B. spp.

Method used

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  • Brevibacillus laterosporus strain S62-9 and application thereof
  • Brevibacillus laterosporus strain S62-9 and application thereof
  • Brevibacillus laterosporus strain S62-9 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Example 1 Brevibacillus sporogenes strain S62-9

[0045] (1) Separation

[0046] The soil (longitude: 120.304, latitude: 31.688) was taken from the hillside of Huishan Mountain in Wuxi, Jiangsu, China. 10 g of soil was mixed with 90 mL of sterile saline to make a suspension by gradient dilution method, and 100 ml of sterile saline was prepared. 5 、10 6 Take 100 μL of each dilution and spread it on a nutrient agar (NA) medium plate, repeat each treatment 3 times, culture the above medium at 37°C for 24 hours, pick a single colony and transfer it to the NA medium plate to streak and purify , to obtain Brevibacillus sporogenes S62-9, and the purified strain was stored in glycerol at -80°C. (2) identification

[0047] After the obtained strain S62-9 was cultured in liquid for 16 hours, the genomic DNA was extracted, and the 16S rDNA gene sequence was amplified by PCR using universal primers. The PCR product was detected by electrophoresis, purified and then sequenced to ...

Embodiment 2

[0051] The microbial inoculant of embodiment 2 Brevibacillus lateralsporosa bacterial strain S62-9

[0052] The present embodiment provides a kind of preparation method of the microbial inoculum of Brevibacillus spp. S62-9, comprising the steps:

[0053] (1) Activation of bacteria

[0054] Inoculate the Brevibacillus sporogenes strain S62-9 with the preservation number CGMCC No.18629 into the nutrient broth medium, 240rpm / min, 37°C constant temperature shaking shaker, culture for 24h, obtain the activated bacterial liquid, and set aside;

[0055] (2) Preparation of seed solution

[0056] The above-mentioned activated bacterial liquid was inoculated in the nutrient broth medium according to the inoculum amount of 3% (V / V), 240 rpm / min, 37 ° C constant temperature shaking shaker, and cultivated for 48 hours to obtain the seed liquid;

[0057] (3) Liquid fermentation

[0058] The seed liquid obtained above was inoculated in the fermentation medium according to the inoculation ...

Embodiment 3

[0062] The microbial inoculant of embodiment 3 Brevibacillus lateralsporosa bacterial strain S62-9

[0063] The present embodiment provides a kind of preparation method of the microbial inoculum of Brevibacillus spp. S62-9, comprising the steps:

[0064] (1) Activation of bacteria

[0065] Inoculate the Brevibacillus sporogenes strain S62-9 with the preservation number CGMCC No.18629 into the nutrient broth medium, 230rpm / min, 37°C constant temperature shaking shaker, culture for 24h, obtain the activated bacterial liquid, and set aside;

[0066] (2) Preparation of seed solution

[0067] The above-mentioned activated bacterial liquid was inoculated into the nutrient broth medium according to the inoculum amount of 3% (V / V), 230 rpm / min, 37 ° C constant temperature shaking shaker, and cultivated for 46 hours to obtain the seed liquid;

[0068] (3) Liquid fermentation

[0069] The seed liquid obtained above was inoculated in the fermentation medium according to the inoculum a...

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Abstract

The invention relates to the technical field of microorganisms, in particular to brevibacillus laterosporus S62-9 and an application thereof. The brevibacillus laterosporus strain S62-9 has the advantages that the brevibacillus laterosporus strain S62-9 is wider in spectrum and more efficient in antibacterial activity, good antibacterial activity is realized on common animal pathogenic bacteria and plant pathogenic bacteria; livestock and poultry pathogenic bacteria which comprise streptococcus suis, salmonella enteritidis, salmonella typhimurium, salmonella paratyphi B, shigella, yersinia enterocolitica, enterobacter sakazakii, klebsiella pneumoniae as well as phytopathogens including fusarium wilt pathogens, root rot pathogens, stem rot pathogens, rice sheath blight pathogens and apple trichothecium roseum heart disease pathogens all have efficient antibacterial activity; the sterilization composition can be widely used for preventing and treating pathogenic bacteria infection of animals such as livestock and poultry and plant crops.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to Brevibacillus spp. S62-9 and its use. Background technique [0002] Brevibacillus laterosporus is a member of the Brevibacillus genus and is a Gram-positive bacterium widely distributed in the natural environment, including plants, animal intestines, seawater, soil and many foods. Since 1989 when Brevibacillus spp. BOD was used as the first human commercial strain, the research on B. spp. has never stopped. In recent years, with the in-depth research on Brevibacillus paraspora, it was found that it can produce a variety of metabolites with potential applications, such as antibacterial substances, insecticidal proteins, etc. For example, in 2012, Zhao Jing et al. reported a strain of Brevibacillus lateralosporus A60 capable of producing antimicrobial peptides. In 2013, Chunglok reported that Brevibacillus lateralosporus SA14 had good effects on methicillin-resistant Staphylococc...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P3/00A23K10/18A23K50/30A23K50/75C12R1/01
CPCA01N63/00A23K10/18A23K50/30A23K50/75C12N1/20C12N1/205C12R2001/01
Inventor 贾英民马爱进韩盼盼
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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