Application and method of oleanane-type pentacyclic triterpene compound in controlling fungal diseases of crops
An oleanane-type, pentacyclic triterpenoid technology, applied in the directions of botanical equipment and methods, applications, fungicides, etc., can solve the problems of undiscovered patent publications, few applications, etc., to improve disease resistance, reduce Disease and resistance improvement effect
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Embodiment 1
[0067] A method for controlling fungal diseases of crops by using oleanane-type pentacyclic triterpene compounds, the steps are as follows:
[0068] Take compound I and prepare 0.5 and 1 g / L solutions with distilled water, respectively. Potato seed potatoes were cut into 3-4 cm square blocks, soaked in two concentrations of compound I solutions respectively, taken out after 1.5 minutes, and placed on the ground to dry naturally for 12 hours. The chemical pesticide control test group soaked potato seed pieces in 80% mancozeb wettable powder (concentration 0.8g / L) solution, took them out after 1.5 minutes, and put them on the ground to let them air naturally for 12 hours.
[0069] The potato seed blocks were sown in flower pots in the greenhouse, and the cultivation substrate was peat, vermiculite and vegetable field soil (ratio 2:1:1), and an appropriate amount of decomposed organic fertilizer was added as the medium, and it was managed routinely. When the potato grows 4-6 lea...
Embodiment 2
[0076] A method for controlling fungal diseases of crops by using oleanane-type pentacyclic triterpene compounds, the steps are as follows:
[0077] Take compound II, and prepare 0.5 and 1 g / L solutions with distilled water, respectively, and set aside.
[0078] Tomato seeds were sown in seedling trays, and the seedling substrate was peat and vermiculite (ratio: 2:1). When the tomato seedlings were 3 to 5 leaves old, the tomato seedlings were taken out, carefully removed the root-adhesive medium matrix, and then immersed in the compound II solution (concentrations were 0.5 and 1 g / L, respectively) for 2 minutes. Tomato seedlings controlled by chemical pesticides were soaked in 80% mancozeb wettable powder (concentration 1.3g / L) solution for 2 minutes.
[0079] Take out the soaked tomato seedlings, spread them on straw mats, let them stand naturally for 0.5 hours, and transplant them into flower pots. The cultivation medium is peat, vermiculite and vegetable field soil (ratio:...
Embodiment 3
[0087] A method for controlling fungal diseases of crops by using oleanane-type pentacyclic triterpene compounds, the steps are as follows:
[0088] Mix compound I and compound II at a ratio of 1:1, prepare 0.5 and 1 g / L solutions with distilled water, and set aside.
[0089] Tomato seeds were sown in seedling trays, and the seedling substrate was peat and vermiculite (ratio: 2:1). When the tomato seedlings reach 3 to 5 leaves, take out the tomato seedlings and transplant them in flower pots. The cultivation medium is peat, vermiculite and vegetable field soil (ratio: 2:1:1). One day after transplanting, the tomato seedlings in the test group were sprayed with the mixed solution of compound Ⅰ and compound Ⅱ to fully moisten the tomato leaves; the chemical pesticide control test group used 80% mancozeb wettable powder (concentration 1.3g / L) Spray control. On the 4th day after transplanting, the prepared tomato early blight pathogenic spore (Alternaria solani) suspension was e...
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