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Photothermal targeted nano medicament for treating breast cancer and preparation method thereof

A breast cancer and drug technology, applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve the problems of low bioavailability, avoid side effects, improve non-specific distribution, and achieve good biophase capacitive effect

Inactive Publication Date: 2020-06-02
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Chinese invention patent application 201910343593.4 discloses a biotin-targeted photosensitizer and quercetin nano-delivery system. Biotin-modified IR780 and quercetin are co-assembled to form a nano-system, which not only solves the problem of quercetin, IR780 Insoluble in water, low bioavailability and other disadvantages, it also overcomes the tumor resistance produced by photosensitizers

Method used

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  • Photothermal targeted nano medicament for treating breast cancer and preparation method thereof
  • Photothermal targeted nano medicament for treating breast cancer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Prepare the fluorescent dye IR783 into 0.5mg / mL~1.5mg / mL aqueous solution, dissolve lapatinib in dimethyl sulfoxide at a concentration of 2.5mg / mL~20mg / mL, and dissolve lapatinib drop by drop under ultrasonic conditions. The dimethyl sulfoxide solution of Tini is added to the IR783 aqueous solution, and then centrifuged at 3,000rpm-7,000rpm for 10 minutes.

[0020] Wash with deionized water for 5 times, and then resuspend with PBS. The average particle size observed under a transmission electron microscope is 80nm-150nm. For details, see figure 1 shown.

Embodiment 2

[0022] The granules prepared in Example 1 were used for killing experiments on breast cancer cells (human breast cancer cells MDA-MB-231, Cell Bank of Chinese Academy of Sciences). The grouping is as follows:

[0023] (1) Control group: no treatment;

[0024] (2) Targeted therapy group: add lapatinib to a final concentration of 2ppm;

[0025] (3) Photothermal group: add IR783 to a final concentration of 10ppm, replace with fresh medium after 4 hours, and irradiate with 1.5W / cm2 808nm laser for 5min;

[0026] (4) Nanomedicine treatment group: add nanomedicine, adjust to an appropriate concentration so that the final system contains lapatinib 2ppm, IR78010ppm, change fresh medium after 4 hours, and use 1.5W / cm 2 808nm laser irradiation for 5min.

[0027] In a 96-well plate, add 100 microliters of 5000 MDA-MB-231 cells per well, and perform the following treatments in each group:

[0028] After culturing for 48 hours, 10 microliters of CCK-8 solution was added to each well. ...

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Abstract

The invention relates to a photothermal targeted nano medicine for treating breast cancer and a preparation method thereof. A composition comprises particles self-assembled from a fluorescent dye IR 783 and lapatinib, wherein the fluorescent dye IR 783 is located on an outer layer of the particles to encapsulate the lapatinib within the particles. The composition is characterized in that a fluorescent dye is arranged on the outer layer of the composition, and a targeted drug is arranged in the composition, so that a problem of poor water solubility of the targeted drug is solved, in vivo membrane penetration of the drug is improved, and killing effect of the drug on cancer cells is improved, meanwhile, a situation of non-specific distribution in vivo of the fluorescent dye is improved, andside effects caused by non-specific distribution are greatly avoided.

Description

technical field [0001] The invention relates to a composition, in particular to a targeted nano-medicine utilizing photothermal effect and its application in breast cancer. Background technique [0002] According to the data of the World Health Organization in 2018, breast cancer accounts for the second (11.6%) of new tumors in the world and the fifth (6.6%) of the deaths caused by tumors. It is worth noting that Asians accounted for 43.6% of the total incidence and 49.6% of the total mortality. Although in the past ten years, many methods have been used for the treatment of breast cancer according to the diagnosis time of breast cancer, such as: surgical resection, radiotherapy, chemotherapy, hormone and biological therapy, but there is still room for improvement in clinical effect. Therefore, the introduction of new and effective breast cancer treatment modalities is urgently needed and necessary. [0003] Chemotherapy alone has many adverse reports, such as chemotherapy...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K31/517A61K9/51A61P35/00
CPCA61K41/0052A61K31/517A61K9/51A61P35/00A61K2300/00
Inventor 贺超郝永强王磊
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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