Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antibacterial peptide TAT-KR-12 resisting infection of airborne bacteria and intracellular bacteria as well as preparation method and application of antibacterial peptide

A technology of planktonic bacteria and antimicrobial peptides, applied in the field of bioengineering, can solve the problems of ineffective resistance to intracellular bacteria and achieve good results, simple process and low cost

Inactive Publication Date: 2020-05-19
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide an antimicrobial peptide against planktonic and intracellular bacteria infection and its preparation method and application, so as to solve the problem that traditional antibacterial drugs cannot effectively resist intracellular bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antibacterial peptide TAT-KR-12 resisting infection of airborne bacteria and intracellular bacteria as well as preparation method and application of antibacterial peptide
  • Antibacterial peptide TAT-KR-12 resisting infection of airborne bacteria and intracellular bacteria as well as preparation method and application of antibacterial peptide
  • Antibacterial peptide TAT-KR-12 resisting infection of airborne bacteria and intracellular bacteria as well as preparation method and application of antibacterial peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The present invention will be further described below in conjunction with the accompanying drawings and the following specific embodiments. It should be understood that the following embodiments and / or accompanying drawings are only used to illustrate the present invention rather than limit the present invention.

[0033] 1. Preparation of peptides:

[0034]Peptides were prepared by standard Fmoc-based solid-phase synthesis techniques on Rink amide MBHA resin. Dicyclohexylcarbodiimide (DCC) and 1-hydroxybenzotriazole (HOBt) were used as coupling reagents, and a 10-fold excess of Fmoc amino acids was added in each coupling cycle. At room temperature with TFA / H2O / thioanisole / phenol / ethanedithiol / triisopropylsilane (81.5:5:5:5:2.5:1, v / v / v / v / v / v After cleavage and deprotection of the mixture of / v) for 2 hours, the crude peptide was repeatedly extracted with diethyl ether and placed on an analytical Vydac C18 column (length: 250mm, inner diameter: 20mm, pore diameter: P...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel antibacterial peptide resisting infection of airborne bacteria and intracellular bacteria and an application of the antibacterial peptide. The novel antibacterial peptide TAT-KR-12 is formed by joining a biological active fragment KR-12 of anthropogenic antibacterial peptide LL-37 and a cell penetrating peptide TAT. The novel synthesized antibacterial peptide TAT-KR-12 has a function of resisting airborne bacteria and has the effect of resisting intracellular bacteria while having good biocompatibility. Besides, the antibacterial peptide has an anti-inflammatoryeffect. The antibacterial peptide is prepared with a simple preparation process, is high in efficiency and good in repeatability, has a good effect on the intracellular bacteria while effectively resisting the airborne bacteria, has certain anti-inflammatory property, makes up the defect of conventional antibacterial drugs and has great clinical significance.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an antibacterial peptide for resisting infection by planktonic bacteria and intracellular bacteria, a preparation method and application thereof. Background technique [0002] Staphylococcus aureus is the most common bacteria in orthopedic infection (Rumian, L., et al., Mater Sci EngCMater Biol Appl, 2016.69: p.856-64.; Wright, J.A. and S.P. Nair, Int J MedMicrobiol, 2010.300 (2 -3): p.193-204.) Staphylococcus aureus can invade host cells including osteoblasts and macrophages, escape the body's immune surveillance and immune clearance, and can survive and reproduce in host cells over time, thereby constituting a reservoir that may lead to recurrent infections (chwarz-Linek, U., M. and J.R. Potts, Mol. Microbiol., 2004.52(3):p.631-41.). Most of the antibiotics currently used clinically have limited ability to act on intracellular bacteria, making it difficult...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K1/107A61K38/17A61K47/64A61P31/04A61P29/00
CPCA61K38/00A61K47/64A61P29/00A61P31/04C07K14/4723C07K2319/10
Inventor 岳冰聂彬恩霍市城
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com