A method for increasing the production of D. spp. mycelium and polysaccharides and application of D. spp. polysaccharides

A technology of mycelium and dry fungus, applied in the biological field, can solve the problems of low yield of dry fungus mycelium and polysaccharide, achieve the effects of no toxicity and side effects, promote growth and polysaccharide formation, and improve the effect

Active Publication Date: 2020-07-14
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the current problem of low production of D. spp. mycelium and polysaccharides, the invention discloses a method for increasing the production of D. spp. mycelium and polysaccharides and the application of polysaccharides. D. s. The yield of exopolysaccharides has been significantly increased, and the extracted ganba polysaccharides not only have antioxidant effects, but also have obvious anti-inflammatory effects

Method used

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  • A method for increasing the production of D. spp. mycelium and polysaccharides and application of D. spp. polysaccharides
  • A method for increasing the production of D. spp. mycelium and polysaccharides and application of D. spp. polysaccharides
  • A method for increasing the production of D. spp. mycelium and polysaccharides and application of D. spp. polysaccharides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Ferment and cultivate the activated D. spp. strain numbered BNCC 230987 in potato dextrose (PDA) liquid medium supplemented with different concentrations of zinc sulfate. Shake culture at 25°C for 7 days. After the fermentation, centrifuge to obtain D. For the filament, wash the mycelium of the dry mushroom 3 times, and dry it at 55°C. Drying bacteria mycelium yield (g / L) = Drying bacteria mycelium mass (g) / culture volume (L). Changes in the mycelium production of Bacillus spp. (BNCC 230987) when the concentration of zinc in the medium increased as follows figure 1 shown;

[0039] (2) Grinding the mycelium of D. spp. obtained in step (1) to obtain powder of D. spp. mycelium, mixing the powder of D. spp. with deionized water at a ratio of 1:20, and adjusting the pH value to 8 , ultrasonic crushing, the ultrasonic power is 300 W, the ultrasonic time is 15 min, and then placed in a 90°C water bath for extraction for 2 hours, and the supernatant is obtained by centri...

Embodiment 2

[0044] (1) Ferment and culture the activated D. spp. strain TG-1 numbered CGMCC No.12977 in potato dextrose (PDA) liquid medium supplemented with different concentrations of zinc sulfate. Shake culture at 25°C for 7 days. After the fermentation , and centrifuge to obtain the mycelia of the dry mushroom, wash the dry mushroom mycelium three times, and dry it at 55° C. to obtain the dried dry mushroom mycelium. Mycelium yield (g / L) = mycelium mass (g) / culture volume (L). Changes in the mycelium yield of S. spp. (CGMCCNo.12977) when the concentration of zinc in the medium increased as follows: Figure 4 shown;

[0045] (2) Grinding the mycelium of D. spp. prepared in step (1) to obtain powder of D. spp. mycelium, mixing the powder of D. spp. with deionized water at a ratio of 1:20, and adjusting the pH value to 8 , ultrasonic crushing, the ultrasonic power is 300 W, the ultrasonic time is 15 min, and then placed in a 90°C water bath for extraction for 2 h, centrifuged to obtain...

Embodiment 3

[0050] Anti-inflammatory activity test

[0051] 72 hpf healthy transgenic Tg(Lyz:GFP) zebrafish with fluorescence were selected, randomly transferred into 24-well plates, and divided into 9 groups, 10 in each group. Among them, the NC group was the normal control group of cultured aquaculture; the MC group was the inflammation model group: the zebrafish were treated with copper sulfate for 2 h. The PC group was the positive control group: indomethacin (5 μg / mL) was co-incubated with zebrafish for 2 h, then treated with copper sulfate (20 μmol / L) for 2 h, and then treated with 4% PFA for 1 h, and cleared Zebrafish were washed with PFA and PBST; Groups I, II, and III were low (25 μg / mL), medium (50 μg / mL) and high dose (100 μg / mL) intracellular polysaccharides of D. Group: zebrafish were co-incubated with 25, 50, and 100 μg / mL mycelial intracellular polysaccharides for 2 h, then treated with copper sulfate (20 μmol / L) for 2 h, and treated with 4% PFA for 1 h, PFA was removed a...

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Abstract

The invention provides a method for improving the yield of thelephora ganbajun mycelia and polysaccharides and application of the thelephora ganbajun polysaccharides. Thelephora ganbajun strains withthe number of BNCC 230987 are subjected to fermented culture in a liquid culture medium with zinc salt, after culture and separation, the thelephora ganbajun mycelia are obtained, endopolysaccharidesof the mycelia are extracted in the thelephora ganbajun mycelia, and exopolysaccharides are extracted in a fermentation liquid obtained after separation of the mycelia. A yield improvement way is provided for producing the thelephora ganbajun mycelia and the polysaccharides by using a liquid fermentation method, when the zinc concentration is 400 mg / L, the yield of the thelephora ganbajun mycelia,the yield of the endopolysaccharides of the mycelia and the yield of the exopolysaccharides are increased by 49.85%, 119.21% and 361.11% respectively, and other thelephora ganbajun strains cannot reach the yield. The thelephora ganbajun polysaccharides prepared by using the method has an anti-inflammatory effect and is free of side and toxic effect, and the thelephora ganbajun polysaccharides canbe developed into health care foods or cosmetics or drugs with anti-inflammatory and anti-oxidative functions.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the production of D. spp. mycelia and polysaccharides and the application of D. spp. polysaccharides. Background technique [0002] Dried bacteria ( Thelephora ganbajun ) is a unique edible fungus in Yunnan, China. It is mainly distributed in pine forests at an altitude of 1000-2200 m. It has a symbiotic relationship with Pinus yunnanensis and Simao pine. Artificial cultivation has not yet been realized. Dried bacteria not only has a strong fragrance, but also contains rich nutrients such as amino acids, proteins, polysaccharides, flavonoids, vitamins, and trace elements in its mycelium, which has high nutritional and health value. Antioxidant substances such as polysaccharides and flavonoids contained in Dried bacterium can remove free radicals in the human body and have the effect of delaying aging; trace elements are beneficial to the preventio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04A23L33/135A61K31/715A61P29/00A61P39/06A61K8/73A61Q19/00A61Q19/08C12R1/645
Inventor 郑岚马耀宏孟庆军公维丽王丙莲杨俊慧杨艳刘庆艾
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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