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Potential application of zinc bacitracin in resisting mycobacterium infection

A technology of bacitracin zinc and mycobacteria, applied in the field of pharmacy, can solve problems such as no report on the application of bacitracin zinc, and achieve significant inhibitory effect and good inhibitory effect.

Inactive Publication Date: 2020-05-19
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the application of bacitracin zinc in anti-mycobacterium tuberculosis infection has not been reported

Method used

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  • Potential application of zinc bacitracin in resisting mycobacterium infection
  • Potential application of zinc bacitracin in resisting mycobacterium infection
  • Potential application of zinc bacitracin in resisting mycobacterium infection

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Embodiment Construction

[0014] In order to better explain the present invention, the specific embodiments of the present invention will be described in detail below.

[0015] 1. Expression and purification of Mycobacterium tuberculosis glycogen branching enzyme (GlgB)

[0016] The gene number of MtbGlgb is Rv1326c and the length is 2196bp. According to the published complete genome sequence of Mycobacterium tuberculosis H37Rv, query the gene sequence of the glycogen branching enzyme of Mycobacterium tuberculosis, and design appropriate primers accordingly, using Mycobacterium tuberculosis H37Rv as a template , PCR amplifies the target gene fragment in vitro. The target gene and Pet-28a vector were digested separately to expose the sticky ends, ligated with T4 ligase, and finally identified and sequenced by digestion to ensure the correctness of the recombinant plasmid. The sequence-correct recombinant plasmid is transferred into competent cells, the transformant is inoculated in liquid LB medium, and ind...

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Abstract

The invention provides an application of zinc bacitracin in resisting mycobacterium infection. A compound can reduce the activity of mycobacterium tuberculosis glycogen branching enzyme, and has a strong inhibition effect on mycobacterium tuberculosis Ra strain at a bacterial level. Therefore, the compound provided by the invention is expected to become an effective inhibitor of mycobacteria.

Description

Technical field [0001] The present invention relates to the technical field of pharmacy, in particular to the application of bacitracin zinc in anti-mycobacterial infection. Background technique [0002] Mycobacteria are divided into three categories: Mycobacterium tuberculosis complex, non-tuberculous mycobacteria and Mycobacterium leprosy. Mycobacterium tuberculosis is the main pathogenic bacterium of tuberculosis. It stays in the cell for a long time and causes latent tuberculosis. If the organism is physiologically inactive for a long period of time, its stored sugar will survive. Becomes very important. Glycogen is one of the most important storage sugars in the biological world, providing nutrients for the host. In addition, glycogen plays a very important role during the interaction between pathogens and hosts. [1] . Therefore, it is important to study the key enzymes in glycogen synthesis. Glycogen is synthesized from UDP-glucose monomer. Glycogen branching enzyme (Gl...

Claims

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Application Information

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IPC IPC(8): A61K38/12A61P31/06
CPCA61K38/12A61P31/06
Inventor 杨海涛黄珍珍刘祥王泽方陈成蔡岩张翅
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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