Method for corn seed priming
A seed and corn technology, applied in the direction of seed immunization, seed and rhizome treatment, botanical equipment and methods, etc., can solve the problems that the expected effect cannot be achieved, and the cold resistance performance of corn cannot be effectively improved, so as to achieve enhanced resistance and prevent germination. Protrusion of roots, effect of preventing mildew
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Embodiment 1
[0062] Embodiment 1 of the present invention discloses a kind of corn seed priming method, comprises the steps:
[0063] (1) Select corn seeds with plump grains and no damage, soak and sterilize them in 0.3% sodium hypochlorite solution for 10 minutes, take out the corn seeds and rinse them with clean water; Air-dried to moisture content <13%;
[0064] (2) Spermidine (Spd) was dissolved in water to prepare an aqueous solution of spermidine with a concentration of 0.25mmol / L, while spermine (Spm) was dissolved in water to prepare an aqueous solution of spermine with a concentration of 0.25mmol / L , and then mixed according to the volume ratio of 1:1 to obtain the treatment solution;
[0065] (3) Arrange 50 corn seeds evenly in each germination box with two layers of filter paper in the germination box, with the embryo facing down, add 0.25mmol L -1 spermidine and 0.25mmol L -1 Add 10 mL of spermine solution to the treatment solution, and add 1 mL of 0.3% NaClO, trigger 72 h a...
Embodiment 2
[0068] Embodiment 2 of the present invention discloses a kind of corn seed priming method, comprises the steps:
[0069] (1) Select corn seeds with plump grains and no damage, add 0.1% sodium hypochlorite solution for soaking and disinfection for 10 minutes, take out the corn seeds and rinse them with clean water; then use filter paper to dry the surface moisture of the corn seeds, and then Air-dried to moisture content <13%;
[0070] (2) respectively dissolving putrescine (Put) and spermine (Spm) in water to prepare a concentration of 0.1mmol / L putrescine aqueous solution and spermine aqueous solution;
[0071] (3) Arrange 50 corn seeds evenly in each germination box with two layers of filter paper in the germination box, with the embryo facing down, add 10mL of 0.1mmol L -1 Putrescine, 10 mL of 0.1 mmol L -1 Each of the spermine solution and 1 mL of 0.1% NaClO was induced for 48 hours at 10°C in the dark;
[0072] (4) Rinse the primed corn seeds with distilled water quick...
Embodiment 3
[0074] Embodiment 3 of the present invention discloses a kind of corn seed priming method, comprises the steps:
[0075] (1) Select corn seeds with plump grains and no damage, add 0.2% sodium hypochlorite solution to soak and disinfect for 10 minutes, take out the corn seeds and rinse them with clean water; then use filter paper to dry the surface moisture of the corn seeds, and then Air-dried to moisture content <13%;
[0076] (2) Dissolving spermidine (Spd) and putrescine (Put) in water respectively to form a concentration of 0.4mmol / L spermidine aqueous solution and putrescine aqueous solution;
[0077] (3) Add 10 mL of 0.4 mmol L to the germination box with two layers of filter paper -1 Spermidine, 10mL of 0.4mmolL -1 Putrescine solution and 1mL of 0.2% NaClO, 50 seeds were uniformly arranged in each germination box, and induced for 70h under dark conditions at 13°C, during which no radicle protruded.
[0078] (4) After the initiation, the seeds were quickly rinsed with...
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