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Compound bacterial agent and its application

A technology of compound bacterial agent and preparation, applied in the direction of application, urea compound fertilizer, bacteria, etc., can solve the problems that need to be studied, and achieve the effect of increasing the weight loss rate of straw, reducing C/N content, and good composting effect of straw

Active Publication Date: 2021-09-21
SINOCHEM AGRI LINYI R&D CENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, microorganisms suitable for straw composting are still to be studied

Method used

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  • Compound bacterial agent and its application
  • Compound bacterial agent and its application
  • Compound bacterial agent and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Strain isolation and screening

[0030] (1) Isolation of cellulase-producing strains: collect rotten straw and livestock manure in the test field of Sinochem Agriculture Linyi R&D Center, weigh 1g into 100ml sterile water, shake at 150rpm, 30°C for 30min, and then carry out gradient diluted to 10 5 , select 3 gradients for coating, and each gradient has 3 parallels. After culturing in a 30°C incubator for 2 days, select strains with different colony forms to streak on LB medium, and observe the growth of colonies regularly. Then, the plate streak method was used to purify the strains, and they were numbered and stored separately.

[0031] (2) Screening of cellulase-producing strains: Prepare LB medium (adding sodium carboxymethyl cellulose) by plate hydrolysis circle method, transplant the strains isolated and preserved in (1) into the center of the plate with a toothpick, and keep at 30°C After incubating at constant temperature for 48 hours, absorb 5ml of 2‰ Cong...

Embodiment 2

[0046] 1. Identification of strain A-2

[0047] (1) Microbiological characteristics: Cultured on LB medium at 37°C for 2 days to form colonies with a size of about 4 mm, neat edges, raised, white viscous and translucent. The optimum growth temperature is 37°C, the salt tolerance (NaCl) concentration is 14%, the growth pH is 4.0-9.0, and the optimum is 7.0.

[0048] (2) Molecular biological characteristics: The phylogenetic tree of the strain formed by the 16SrDNA sequence of molecular biology showed that the strain had the closest homology to Bacillus siamensis strain XSG14. The strain was Bacillus siamensis.

[0049] The 16SrDNA sequence of the strain is as follows, and the phylogenetic tree can be found in figure 1 .

[0050] TCTCGTGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATG CTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCC GAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTC TGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATC CCCACCTTCCTCCG...

Embodiment 3

[0056] The preparation of embodiment 3 microbial bacterial agents

[0057] 1. Preparation of Bacillus siamese preparation

[0058] Step 1: Inoculate the Bacillus siamese strain A-2 preserved in the plate into an Erlenmeyer flask containing 250ml of LB culture solution with an inoculation loop, and culture it in a constant temperature incubator at 30°C and 170rpm for 36h.

[0059] Step 2: Inoculate the above-mentioned fermented bacteria liquid into a pilot test fermenter (10L-50L-500L), cultivate at 37°C, spray dry after the fermentation is completed, and subpackage. The number of viable bacteria is 10-15 billion / g. Mix it with calcium carbonate to form a bacterial powder with a viable count of 10 billion / g, which is to prepare the Bacillus siamese A-2 bacterial agent for composting. The formula of pilot test medium is: corn starch 3%, sucrose 1%, soybean meal 4.5%, yeast powder 0.2%, peptone 0.2%, dipotassium hydrogen phosphate, potassium dihydrogen phosphate 0.3%, calcium ca...

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Abstract

The present invention proposes a composite bacterial agent and its application. The composite bacterial agent includes: Aspergillus awamori preparation and Bacillus siamese preparation, wherein the Aspergillus awamori in the Aspergillus awamori preparation was preserved in The General Microbiology Center of the China Committee for the Preservation of Microbial Cultures, the preservation number is CGMCC No.18595, and the Bacillus siamese in the Bacillus siamese preparation was preserved in the General Microbiology of the China Committee for the Preservation of Microbial Cultures on October 15, 2019 Center, the deposit number is CGMCC No.18688. The composite bacterial agent of the present invention has a better ability to decompose cellulose, can be effectively applied to straw composting, obviously accelerates the temperature rising speed in the early stage of composting, increases the fermentation temperature, increases the weight loss rate of straw after composting, reduces the C / N content, and makes composting more efficient. The degree of maturity is high, suitable for large-scale application.

Description

technical field [0001] The present invention relates to the field of biology. Specifically, the present invention relates to a composite microbial agent and its application. Background technique [0002] With the advancement of farming technology, the output of straw is increasing. However, due to the low utilization rate of straw, most of it is burned, which leads to waste of resources and environmental pollution. As a good raw material for organic fertilizer production, straw has not been fully utilized due to the lack of mature and efficient conversion technology. Returning the straw to the field can make full use of the nutrient resources in the straw and improve the soil. After returning to the field, the straw decomposes under the action of soil microorganisms, and the cellulose, hemicellulose, and lignin in the straw are converted into simple compounds that are easily absorbed by plants, thereby improving the physical and chemical properties of the soil, increasing ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/20C05F11/00C05G1/00C05F17/20C12R1/665C12R1/07
CPCC05C9/00C05F11/00C12N1/14C12N1/20Y02W30/40
Inventor 邱鹏飞李洪顺张西兴李延锋柴彦亮
Owner SINOCHEM AGRI LINYI R&D CENT CO LTD
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