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Potential application of closantel sodium in resisting mycobacterium infection

A kind of technology of cylindronate sodium and mycobacteria, which is applied in the field of pharmacy and can solve the problems such as no report on the application of cyhalothalide sodium.

Inactive Publication Date: 2020-05-15
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the application of Closantamide Sodium in anti-mycobacterial infection has not been reported.

Method used

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  • Potential application of closantel sodium in resisting mycobacterium infection
  • Potential application of closantel sodium in resisting mycobacterium infection
  • Potential application of closantel sodium in resisting mycobacterium infection

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Embodiment Construction

[0014] In order to better illustrate the present invention, specific embodiments of the present invention will be described in detail below.

[0015] 1. Expression and purification of anthranilate phosphoribosyltransferase (AnPRT) in mycobacteria

[0016] (1) Transform the recombinant plasmid containing the gene encoding AnPRT into Escherichia coli BL21(DE3).

[0017] (2) Pick positive clones on the plate and expand the expression after culturing overnight.

[0018] (3) Autoclave the bacteria after collecting the cells; collect the supernatant.

[0019] (4) Add the supernatant to a Ni-NTA affinity chromatography column, and use Ni-NTA affinity chromatography to obtain a relatively uniform protein.

[0020] (5) Purify by gel filtration chromatography to further obtain the target protein with charge uniformity.

[0021] 2. Activity determination of AnPRT

[0022] Sodium anthranilate with a purity greater than 95% was used as a substrate; the kinetic curve was determined by a...

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Abstract

The invention discloses potential application of closantel sodium in resisting mycobacterium infection, and provides an inhibitor of an enzyme which exists in mycobacterium and is very important for growth of the mycobacterium, namely, anthranilate phosphoribosyl transferase (AnPRT). The inhibitor is named closantel sodium and has significant inhibition activity for transfer of the AnPRT, so thatthe inhibitor can be used for preparing a small-molecular inhibitor for the AnPRT in the mycobacterium and is expected to become a potential drug for resisting mycobacterium infection.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to the application of iodosan sodium in anti-mycobacterial infection. Background technique [0002] Mycobacterium (Mycobacterium, mycobacterium) is currently classified as actinomycetes, generally in the shape of a curved long rod, sometimes branched or filamentous. There are many types of mycobacteria, including non-tuberculous mycobacteria and tuberculosis mycobacteria. Among them, Mycobacterium tuberculosis (Mycobacterium tuberculosis, Mycobacterium tuberculosis) is the main pathogenic bacterium of tuberculosis, and it is transmitted as aerosol through the lung route, and only a few bacilli are needed to establish infection. After infection, Mtb can survive long-term in the granulomas that form in the lung [1] . Chemotherapy can be used to treat TB, but in the best case, a multidrug regimen of at least 6 months is required to successfully treat the infection. In many cases, ...

Claims

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Application Information

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IPC IPC(8): A61K31/277A61P31/04
CPCA61K31/277A61P31/04
Inventor 杨海涛常晓娟刘祥王泽方陈成蔡岩黄珍珍
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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