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Multilayer structure stent as well as preparation method and application

A multi-layered, three-dimensionally structured technology with applications in biochemical devices and methods, cell culture supports/coatings, microorganisms, etc.

Active Publication Date: 2020-05-12
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still challenges in the development of large-scale in vitro expansion systems, that is, there is still a lack of an expansion system that can simultaneously satisfy the following conditions: 1) a large number of cells are absorbed and evenly distributed throughout the culture system; 2) there are sufficient nutrients Transportation, mechanical stability can support long-term culture; 3) Cells / cell clusters after harvest maintain their phenotype and function

Method used

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  • Multilayer structure stent as well as preparation method and application
  • Multilayer structure stent as well as preparation method and application
  • Multilayer structure stent as well as preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0088] Example 1 Preparation of multi-level structure stent by casting method

[0089] This embodiment provides a multi-level structure support, such as figure 1 As shown, the multi-level structure stent includes a stent body, wherein the interior of the stent body has large through-holes with an average pore diameter of about 100 μm, the porosity of the stent body is 75%, and the Young’s modulus of the stent body is is 220kPa; the height of the stent body is 6cm, and the diameter (referring to the outer diameter) is 6cm. The stent body also has a hollow channel, and the diameter of the channel is 2cm.

[0090] This embodiment provides a method for preparing the above-mentioned multi-level structure support, including the following steps:

[0091] 1. Preparation of Biomaterial Solution

[0092] 21% polyglycolic acid solution: mix polyglycolic acid powder (Sigma-Aldrich) with 0.9% sodium chloride solution at a mass ratio of 21:100, stir with a magnetic stirrer for about 5 min...

Embodiment 2

[0129] Example 2 Preparation of a scaffold with a multi-level structure by three-dimensional printing with a single nozzle

[0130] This embodiment provides a multi-level structure support, such as Figure 4 , Figure 5 As shown, the multi-level structure stent includes a stent body, wherein the interior of the stent body has through macropores with an average pore diameter of about 100 μm, the porosity of the stent body is 95%, and the Young’s modulus of the stent body is 30kPa; the stent body has a three-dimensional structure with a size of 1cm×1cm×0.5cm, the stent body is composed of material filaments of about 300 μm, and the stent body has hollow channels with an interval of about 1cm.

[0131] Further, the stent body is composed of multiple levels of structure.

[0132] This embodiment provides a method for preparing the above-mentioned multi-level structure support, including the following steps:

[0133] 1. Prepare a precursor solution with a concentration of 7% pol...

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Abstract

The invention relates to a multilayer structure stent as well as a preparation method and application. The multilayer structure stent comprises a structure from a centimeter scale to a micrometer scale, and is applied to the fields of 3D cell culture, in vitro large-scale amplification, in vitro tissue-like construction, tissue engineering and regenerative medicine, pathological model research, new drug research and development and drug toxicology research. The multilayer structure stent has the characteristics of customized macrostructure, controllable hierarchical structures, adjustable poresize, high porosity and permeability, high cell load, high elasticity modulus, good mechanical properties, good cell functions and nondestructive cell collection.

Description

technical field [0001] The invention relates to the technical field of biological tissue engineering, in particular to a multi-layer structure scaffold and its preparation method and application. Background technique [0002] In general, a single dose of adult cell transplantation must reach 10 8 ~10 9 cells to function effectively. At the same time, stem cell therapy products must meet safety, efficacy and minimum batch-to-batch variation to ensure stable therapeutic effects. Therefore, cost-effective strategies that enable the expansion, differentiation, and / or functional maintenance and harmless harvest of stem cells are urgently needed. [0003] Compared with planar culture, three-dimensional (3D) cell culture can reduce the difference between in vitro culture and native tissue by recreating cell-cell and cell-matrix interactions, which has great advantages. For micron-scale culture systems, microcarriers have been used as platforms for large-scale expansion of mesen...

Claims

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Application Information

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IPC IPC(8): C12N5/00
CPCC12N5/0062C12N2535/00C12N5/0068
Inventor 姚睿
Owner TSINGHUA UNIV
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