Biological lysis treatment method for sludge

A treatment method and sludge technology, applied in biological sludge treatment, sustainable biological treatment, biological water/sewage treatment, etc., can solve the problems of complicated operation, limited use conditions, expensive enzymes, etc., and achieve fast dissolution rate, The effect of saving carbon sources

Pending Publication Date: 2020-05-12
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The lysis method using strong acid and strong alkali can achieve a considerable degree of sludge dissolution, but considering the cost of chemicals and subsequent waste liquid treatment and possible safety issues for workers, this cannot be used as a practical application ideal method
[0006] As a biological method, the use of biological enzymes to decompose solid organic components in sludge has been proposed. Although the operating conditions of this method are mild, the enzymes used are very expensive, so large-scale application is very difficult, and according to different The characteristics of the enzyme and the conditions of use are strictly limited, which makes the operation more complicated

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  • Biological lysis treatment method for sludge
  • Biological lysis treatment method for sludge
  • Biological lysis treatment method for sludge

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Domestication of seed mud

[0040] The sludge used for domestication comes from the residual sludge of the sewage plant operated by A / O process. The total suspended solids (TSS) in the sludge are 10147±210mg / L, and the volatile suspended solids (VSS) are 7416±170mg / L. A laboratory-scale thermophilic aerobic reactor (R 55 : 55°C; R 65 : 65°C; R 75 : 75° C.), the reactor was combined with a heat-collecting magnetic stirrer (150 rpm), and the volume of each reactor was 1 L. After the sludge is sieved to remove substances with a diameter of more than 1mm, it is added to the reactor, which is the domestication stage of the seed sludge. 1 / 5 volume of domesticated sludge in the reactor was replaced with fresh sludge every 3 days to provide nutrition for thermophilic bacteria, and the domestication time was 15 days.

[0041] Protein is the main component in sludge, and the hydrolysis of protein is an important part of the process of dissolving sludge. The method of measurin...

Embodiment 2

[0043] Sludge Dissolution Experiment

[0044] The analysis experiment of the thermophilic flora formed under the above three temperature conditions on the sludge dissolving performance is as follows.

[0045] Inoculate 5% of the domesticated seed sludge containing thermophilic bacteria in the sludge dissolving reactor to carry out the sludge dissolving experiment, and use three reactors without thermophilic bacteria as the control group, the control group and the experimental group respectively. The operating temperature is the same, and the operating conditions are the same as those in the acclimation stage. Samples were taken at different time points during the operation of the reactor, and the position of the liquid level in the reactor was recorded before and after sampling, and the evaporated water was replenished with distilled water. The concentration of VSS in solution at different time points was detected according to standard methods. The method for measuring the s...

Embodiment 3

[0049] Microbial population structure and function

[0050] Thermophilic communities in all reactors were assessed using Illumina amplicon sequencing. Sludge samples were collected from three acclimatization reactors that had been in operation for 15 days, and DNA samples were extracted from the sludge using the Fast DNA Spin Kit for soil (MP Biomedicals, LLC, USA). DNA concentration was then measured using NanoDrop (ND-1000, DE, USA). Bacterial 16S rRNA gene was PCR amplified with forward primer 515F and reverse primer 806R. And pyrosequencing was performed on the Illumina HiSeq platform.

[0051] A total of 605,480 cleantags were generated from 9 samples (three biological parallels) by Illumina HiSeq sequencing. After removing chimeric sequences, the effective tags were clustered into taxonomic operating units (OTUs) with 97% similarity, Deinococcas-Thermus phylum (Deinococcas-Thermus ) in the genus Thermus as the dominant genus in R55, R65 and R75 relative abundances wer...

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Abstract

The invention discloses a method for quickly lysing sludge by utilizing thermophilic bacteria. A thermophilic microbial population includes the following species that secrete hydrolases capable of dissolving sludge: bacteria of thermus and geobacillus sp. and thermophilic aerobic bacteria. The hydrolases secreted by the thermophilic bacteria comprise proteolytic enzyme, polysaccharide hydrolase and peptidoglycan hydrolase, so the floc structure of the sludge can be quickly hydrolyzed. The specific method comprises the operation step that with sludge as a matrix, and potential thermophilic bacteria in the sludge are revived through long-time domestication under a heating condition to form a microbial preparation containing a stable thermophilic bacterial group. The microbial preparation disclosed by the invention can be used for treating biological sludge discharged from sewage treatment plants, chemical plants, food factories and the like, and rapid dissolution of the sludge can be realized through the synergistic effect of various hydrolases generated by the thermophilic bacteria. When the method is used for dissolving the sludge, a device is simple in structure, small in occupiedarea, easy to operate and low in energy consumption.

Description

technical field [0001] The invention belongs to the biological treatment technology of sludge, in particular to a biological lysis treatment method of sludge. Background technique [0002] The activated sludge process is currently widely used in the treatment of domestic sewage and organic industrial wastewater. Its main disadvantage is that it produces a large amount of excess activated sludge. With the increase of the population and the increasing emphasis on environmental protection, the amount and rate of sewage treatment continue to increase, and the amount of sludge produced is also increasing day by day. Traditional landfill, incineration and other sludge treatment technologies are increasingly restricted due to their defects such as large land occupation, secondary pollution and high pretreatment costs. Therefore, the treatment and disposal of sludge has become a major problem in the environmental field. In-situ sludge reduction is an important means to dispose of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/02C02F3/12C02F101/30
CPCC02F3/12C02F11/02C02F2101/30Y02W10/10
Inventor 黄瑛迟宝岩袁领娓
Owner SOUTHEAST UNIV
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