Inorganic-organic hybrid Keggin type polyoxometallate compound as well as preparation method and antibacterial application thereof

A technology of polyoxometalates and organic hybridization, which is applied in organic chemistry, antibacterial drugs, organic active ingredients, etc., can solve the problems of instability of pure inorganic substances, limit cell penetration, etc., and achieve enhanced stability and Penetration ability, excellent antibacterial performance, strong stability effect

Active Publication Date: 2020-05-08
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, pure inorganics exhibit instability under physiological conditions and limit cell penetration due to their surface properties

Method used

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  • Inorganic-organic hybrid Keggin type polyoxometallate compound as well as preparation method and antibacterial application thereof
  • Inorganic-organic hybrid Keggin type polyoxometallate compound as well as preparation method and antibacterial application thereof
  • Inorganic-organic hybrid Keggin type polyoxometallate compound as well as preparation method and antibacterial application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method of an inorganic-organic hybrid Keggin type polyoxometalate compound, comprising the steps of:

[0036] Will Co(NO 3 ) 2 ·6H 2 O (0.189 g, 1.3 mmol), Na 2 MoO 4 2H 2 O (0.48 g, 2.0 mmol), EDTMP (0.35 g, 0.8 mmol) and 2-acetylpyrazine thiosemicarbazone (0.097 g, 0.5 mmol) were added to 60 mL of water and methanol (volume ratio 2:1) In the mixed solvent, stir for 40 minutes until completely dissolved, adjust the pH range between 2.5–3.0 with dilute hydrochloric acid, then transfer to a reaction bottle, put it in a polytetrafluoroethylene liner, keep it at 130°C for 3 days, and cool to room temperature, washed with water, and dried to obtain black tetragonal crystals, which is the complex.

[0037] The above-mentioned inorganic-organic hybrid Keggin type polyoxometalates belong to the monoclinic crystal system, C2 / c The space group, whose unit cell parameters are: a = 29.1829(13) Å, b = 16.5031(7) Å, c = 18.9095(9) Å, α = 90°, β = 103.46...

Embodiment 2

[0047] Antibacterial effect evaluation method 1 disc diffusion method:

[0048] Using DMSO as the blank control group, the antibacterial activity of the material was studied by means of an agar plate test. Before the test, the complex was diluted with DMSO in a gradient (1 mg / mL, 0.5 mg / mL, 0.25 mg / mL, 0.125 mg / mL, 0.0625 mg / mL, 0.03125 mg / mL...). All materials used during testing were autoclaved and nutrient agar plates (NA) were sterilized by UV radiation for 1 h. will be 10 7 CFU / mL of Staphylococcus aureus ( S. aureus ), Bacillus subtilis ( B. subtilis ) and 10 6 Escherichia coli in CFU / mL ( E. coli ) and Agrobacterium tumefaciens ( A. tumefaciens) fresh bacteria solution were uniformly spread on the nutrient agar (NA) plate, and then punched, the prepared complexes were sucked into the wells of the agar plate in turn, and then sealed, and the culture was incubated at 37°C for 16 hours , and finally, the size of the zone of inhibition (ZOI) of all samples was me...

Embodiment 3

[0054] Time killing test evaluation 2 colony forming unit (CFU) method:

[0055] The bactericidal ability of the compound was studied by plate coating culture method. The E. coli and S. aureus solutions were serially diluted to 10 5 CFU / mL, and prepare a 1 mg / mL complex solution. Equal volumes of the inoculated bacteria solution and the complex solution were incubated at 37°C for different times, and the bacteria solution without the complex was used as a control experiment. The assay was repeated three times and cultured for 16 hours in a constant temperature incubator at 37°C. The experiment was continued until almost no colonies were observed. The experimental results show as Figure 9 with 10 . Bacterial cell survival rate (%) = (number of colonies in the experimental group / number of colonies in the control group) × 100%.

[0056] Figure 9 with Figure 10 The complex pairs of the present invention are given respectively E. coli with S. albus time-killing pic...

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Abstract

The invention discloses an inorganic-organic hybrid Keggin type polyoxometallate compound; the chemical formula of the compound is [Co(L)2]3[PMo12O40], and in the formula, HL is 2-acetylpyrazine thiosemicarbazone; the compound belongs to a monoclinic system and a C2 / c space group, and cell parameters comprises a=29.1829(13) angstroms, b=16.5031(7) angstroms, c=18.9095(9) angstroms, alpha=90 degrees, beta=103.465(1) degrees and gamma=90 degrees. According to the compound, the 2-acetylpyrazine thiosemicarbazone and the POM are self-assembled together; the stability and penetrability of the POM are enhanced, the POM has excellent antibacterial performance, and the obtained compound can play a considerable antibacterial role in the biological and food industries and other application fields, for example, the compound can be used as an antibacterial material and an antibacterial reagent or used for preparing antibacterial drugs and the like.

Description

technical field [0001] The invention belongs to the technical field of inorganic-organic functional polyacid. It specifically relates to a combination of 2-acetylpyrazine thiosemicarbazone compound with good bactericidal effect and POM with high redox, anticancer and antimicrobial activities to form an inorganic-organic hybrid Keggin-type polyoxometallic acid salt complex. The complex self-assembles 2–acetylpyrazine thiosemicarbazone with POM, which not only enhances the stability and penetration ability of POM, but also makes it have excellent antibacterial properties, which can be used in biological and food industries and other applications. Fields exert a considerable antibacterial effect. Background technique [0002] Bacterial infections are a serious problem that can threaten human health and the growth of plants and animals. Overuse or even misuse of antibiotics can make bacteria resistant to antimicrobial drugs. Today, millions of people all over the world die f...

Claims

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Application Information

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IPC IPC(8): C07D241/12A61K31/4965A61P31/04A61K33/24A61K47/55
CPCC07D241/12A61P31/04A61K33/24A61K47/55
Inventor 李明雪赵萌刘泰宇方颜刘静
Owner HENAN UNIVERSITY
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