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Method for detecting concentration of metal elements in aqueous humor in combination with flow injection and inductively coupled plasma mass spectrometer

A metal element and inductive coupling technology, applied in the field of medicine, can solve the problem of low sensitivity, achieve the effect of reducing wall hanging, eliminating matrix effect and signal drift

Active Publication Date: 2020-05-01
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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Problems solved by technology

[0007] The matrix effect of ICP-OES is smaller than that of ICP-MS, but its sensitivity is lower than that of ICP-MS, and ultra-trace analysis still needs to use ICP-MS method

Method used

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  • Method for detecting concentration of metal elements in aqueous humor in combination with flow injection and inductively coupled plasma mass spectrometer
  • Method for detecting concentration of metal elements in aqueous humor in combination with flow injection and inductively coupled plasma mass spectrometer
  • Method for detecting concentration of metal elements in aqueous humor in combination with flow injection and inductively coupled plasma mass spectrometer

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Embodiment 1

[0029] 1. Collection and storage of aqueous humor

[0030] During the operation, routine disinfection and draping are performed. Before the invasive operation of the anterior chamber, the anterior chamber puncture is performed with a disposable sterile 1mL syringe, and 100-150 μl of aqueous humor samples are collected and transported to the laboratory in an ice box , transferred to a 1.5ml sterilized EP tube under aseptic conditions in a biological safety cabinet, sealed with parafilm, quickly frozen in liquid nitrogen, and stored in a -80°C refrigerator. The aqueous humor samples were uniformly sent to the Sun Yat-Sen University Testing Center for testing by FI-ICP-MS method, and each sample was divided into two parallel samples for testing, and finally the average value of the two measurements was taken. Single-blind operation was adopted.

[0031] 2. Pretreatment of aqueous humor detection

[0032] A. Take 50 μl aqueous humor sample in a 1.5mL sterilized EP tube (for the ...

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Abstract

The invention discloses a method for detecting concentration of metal elements in aqueous humor in combination with flow injection and an inductively coupled plasma mass spectrometer. The method comprises the following steps of first, taking an aqueous humor sample; then, diluting the aqueous humor sample, and adding Triton, methanol, HNO3 and internal standard elements to obtain a diluent; and finally, injecting, by using the flow injection, the sample into the inductively coupled plasma mass spectrometer for determining the concentration of the metal elements to be detected. According to themethod, aqueous humor detection pretreatment is adopted for solving the problem that the aqueous humor sample has a wall hanging phenomenon in a sample injecting pipeline, so that the wall hanging ofthe sample in the sample injecting pipeline is greatly reduced. Moreover, by adding the internal standard elements, namely, manually selecting and adding the elements, namely, Sc, In and Ge, that arevery rare in the natural environment, the solution sample volume is standardized, and a matrix effect and signal drift are eliminated. In addition, the values to be set of the relevant parameters ofan instrument are found out, the concentration of trace metal elements in the aqueous humor is successfully detected by applying FI-ICP-MS for the first time, and taking Zn as an example, the detection limit is as low as 2.00 ug / L. The method for detecting the concentration of the metal elements in the aqueous humor in combination with the flow injection and the inductively coupled plasma mass spectrometer provides a model for the detection of the trace metal elements in the aqueous humor.

Description

Technical field: [0001] The invention belongs to the field of medicine, and in particular relates to a method for detecting the concentration of aqueous humor metal elements by combining flow injection and inductively coupled plasma mass spectrometer. Background technique: [0002] Trace metal elements usually refer to metal elements below 0.01% of the body weight. Although the content of trace metal elements in the human body is extremely small, they play important physiological and pathological functions and are one of the indispensable substances for the human body. The essential trace metal elements that have been confirmed to be related to human health and life include iron, copper, zinc, cobalt, manganese, chromium, nickel, molybdenum, vanadium, tin, strontium, rubidium, etc. [0003] Studies have shown that the concentrations of various trace metal elements are changed to varying degrees in many diseases. For example, in the intraocular fluid of patients with age-re...

Claims

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Application Information

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IPC IPC(8): G01N27/62G01N1/38G01N27/626
CPCG01N27/62G01N1/38
Inventor 李轶擎卓业鸿李杨佳旎刘洪涛梁舒欣
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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