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Application of epicannabidiol hydrate in preparing drugs for preventing and/or curing brain injuries and pharmaceutical composition comprising epicannabidiol hydrate

A technology of epi-cannabidiol hydrate and brain injury, applied in the field of natural medicine and drug treatment, can solve the problem that the treatment effect of brain injury disease has not been reported, and can reduce the volume of cerebral infarction, reduce the volume of cerebral infarction, and reduce brain injury. Effect

Active Publication Date: 2020-05-01
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the therapeutic effect of epicannabidiol hydrate on brain injury diseases mediated by brain inflammation has not been reported

Method used

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  • Application of epicannabidiol hydrate in preparing drugs for preventing and/or curing brain injuries and pharmaceutical composition comprising epicannabidiol hydrate
  • Application of epicannabidiol hydrate in preparing drugs for preventing and/or curing brain injuries and pharmaceutical composition comprising epicannabidiol hydrate
  • Application of epicannabidiol hydrate in preparing drugs for preventing and/or curing brain injuries and pharmaceutical composition comprising epicannabidiol hydrate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Determination of cellular anti-inflammatory pharmacological activity:

[0043] (1) Cytotoxicity assay:

[0044] Dissolve epicannabidiol hydrate in dimethyl sulfoxide (DMSO) to prepare a solution with a concentration of 0.1, 1, 2, 5, 10, 20, and 50 μM, and add 100 μL 5000 to each well of a 96-well cell culture plate RAW264.7 cells or primary mouse microglial cells were added with 10 μL epicannabidiol hydrate solution of each concentration for 24 hours, and then 10 μL CCK-8 solution was added to each well, and the culture was continued for 2 hours, and measured at 450 nm Absorbance.

[0045] Test results such as figure 1 A and figure 1 As shown in B, where * P** P<0.01, vs Control, (Graphpad6.0, One-way Anova). It can be seen from the figure that epicannabidiol hydrate has no significant toxicity to RAW264.7 cells or primary mouse microglia at a concentration of 10 μM or below.

[0046] (2) Determination of cells releasing inflammatory factor proteins:

[0047] RAW...

Embodiment 2

[0069] Improvement effect of epicannabidiol hydrate on ischemic stroke

[0070] (1) Experimental grouping

[0071] Mice were randomly divided into 4 groups, 8 in each group, respectively sham operation group, ischemia-reperfusion model group (model group), compound treatment low-dose group (low-dose group, 1mg / kg) and compound treatment high-dose group (high dose group, 10mg / kg).

[0072] (2) Establishment of mouse transient middle cerebral artery occlusion model (tMCAO)

[0073] Place the mouse in an anesthesia induction box, give 2-2.5% isoflurane to induce anesthesia, take it out after the righting reflex disappears, put on a breathing mask, give 1-1.5% isoflurane to maintain anesthesia, and use Doppler After measuring the blood flow of the right brain with a blood flow meter, fix the mouse in the supine position, prepare the skin on the neck, disinfect with iodine, expose the right common carotid artery, external carotid artery and internal carotid artery, hang the threa...

Embodiment 3

[0091] Ameliorative effect of epicannabidiol hydrate on lipopolysaccharide-induced neuroinflammation in mice

[0092] (1) Experimental grouping and dosing regimen

[0093] The mice were randomly divided into 4 groups, 8 in each group, which were blank control group, model group, low-dose compound treatment group (low-dose group) and high-dose compound treatment group (high-dose group). 48h and 24h before modeling, epicannabidiol hydrate DMSO solution (1mg / kg, 10mg / kg) was injected into the tail vein, and the administration volume was 4mL / kg. The blank control group and the modeling group were injected with the same amount of DMSO .

[0094] (1) Establishment of a mouse neuroinflammation model induced by lipopolysaccharide

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Abstract

The invention discloses application of epicannabidiol hydrate in preparing drugs for preventing and / or curing brain injuries and a pharmaceutical composition comprising the epicannabidiol hydrate. According to the application of the epicannabidiol hydrate in preparing the drugs for preventing and / or curing the brain injuries, it is the first time to discover that the epicannabidiol hydrate has a drug effect of obviously relieving a brain injury caused by a cerebral ischemic stroke. Researches show that the epicannabidiol hydrate relieves in vitro and vivo neural inflammatory reactions by inhibiting expression of a proinflammatory factor and promoting expression of an anti-inflammatory factor, has a function of obviously decreasing the volume of cerebral infarction after transient middle cerebral artery occlusion, relieves inflammatory overreactions of ischemic brain tissue, and realizes a neuroprotective effect after cerebral ischemia, and can be effectively applied to preventing and / or curing the brain injuries, especially the brain injury caused by the cerebral ischemic stroke; and the pharmaceutical composition comprising the epicannabidiol hydrate can become a novel drug for preventing and / or curing the brain injuries of brain diseases closely associated with brain inflammations, and various brain diseases can be cured.

Description

technical field [0001] The invention belongs to the field of natural medicine and medicine treatment. , specifically relates to the application of epicannabidiol hydrate in the preparation of drugs for preventing and / or treating brain damage and its pharmaceutical composition. Background technique [0002] Cerebrovascular disease is one of the three diseases with the highest mortality rate in the world. Ischemic stroke is one of the most serious diseases among cerebrovascular diseases. It is one of the most important problems that seriously endanger human health and life safety in the world today. It is characterized by high morbidity, high disability, high recurrence and high mortality. my country is a big country with cerebrovascular diseases. About 2 million people suffer from stroke every year, and ischemic stroke patients account for about 75% to 85%. China spends more than 10 billion yuan on the treatment of stroke every year, which brings a heavy economic burden to ...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P9/10
CPCA61K31/05A61P9/10
Inventor 庞涛河泰麟姜其慧张毅楠张陆勇王浩杰
Owner CHINA PHARM UNIV
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