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Copper phthalocyanine molecule-based diagnosis and treatment integrated nanoprobe as well as preparation and application thereof

A nano-probe, copper phthalocyanine technology, applied in the field of biomedicine, can solve the problems of poor solubility, low molecular selectivity, detection and treatment method limitations, etc., and achieve the effect of small particle size

Active Publication Date: 2020-04-28
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional photosensitive molecules have defects such as poor solubility and low molecular selectivity, which limit their use as detection and treatment methods

Method used

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  • Copper phthalocyanine molecule-based diagnosis and treatment integrated nanoprobe as well as preparation and application thereof
  • Copper phthalocyanine molecule-based diagnosis and treatment integrated nanoprobe as well as preparation and application thereof
  • Copper phthalocyanine molecule-based diagnosis and treatment integrated nanoprobe as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 Preparation of integrated probe for diagnosis and treatment based on copper phthalocyanine molecule

[0084] (1) Disperse 1 g of original hexagonal boron nitride powder in a certain amount of concentrated sulfuric acid under magnetic stirring. 0.5g potassium permanganate (KMnO 4 ) was slowly added into the mixing system, and continued stirring for 12 hours. Then add 10mL of analytically pure hydrogen peroxide (H 2 o 2 ). The resulting suspension was sonicated for 1 hour and then centrifuged at 10000 rpm for 10 minutes to remove large h-BN powder. The supernatant was washed with ultrapure water until the pH of the filtrate was neutral. Finally, the product was placed in a vacuum oven and dried at 60 °C for 24 hours to obtain hexagonal boron nitride nanosheets (h-BNNS).

[0085] The particle size range of the hexagonal boron nitride nanosheets (h-BNNS) is 80-100 nm, and the thickness is about 15 nm. figure 1 A is the transmission electron microscope figur...

Embodiment 2

[0089] The nanoprobe prepared in Example 1 is used for the diagnosis and treatment of tumors, the specific method is as follows:

[0090] (1) Disperse the nanoprobe (CuPc@HG@BN) in phosphate buffer so that the final concentration of the probe is 100 μg / ml. Then the probes were reacted with 0.1fM-20pM microRNA-21 for 1 hour, and then the Raman intensity of the solution was measured with an excitation wavelength of 780nm. figure 2 is the Raman spectrum and calibration curve of the nanoprobe (CuPc@HG@BN) in response to different concentrations of microRNA-21, from figure 2 In a, it can be seen that as the concentration of microRNA-21 increases, the characteristic peak of CuPc molecule is 1530cm -1 The Raman intensity at the position decreases gradually, and the characteristic peak of h-BNNS is 1367cm -1 The Raman intensity at remains constant through I 1530 / I 1367 The linear relationship with the logarithm of the microRNA-21 concentration results in a detection limit of 0....

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Abstract

The invention relates to a copper phthalocyanine molecule-based detection, imaging and treatment integrated nanoprobe. The copper phthalocyanine molecule-based detection, imaging and treatment integrated nanoprobe comprises hexagonal boron nitride nanosheets, copper phthalocyanine molecules and two groups of oligonucleotide binding sequences. One end of each of the two groups of oligonucleotide sequences designed by the invention is a base pair for detecting and circularly amplifying microRNA-21, and the other end is an AS1411 sequence. The AS1411 sequence not only can recognize nucleolin highly expressed on the surfaces of tumor cells to play a role in targeted localization, but also can carry copper phthalocyanine molecules with poor water solubility based on a G quadruplex formed underspecific conditions, then, the nucleotide sequence loaded with copper phthalocyanine is adsorbed on the surfaces of the hexagonal boron nitride nanosheets through pi-pi accumulation to enter tumor cells. The invention further discloses application of the copper phthalocyanine molecule-based detection, imaging and treatment integrated nanoprobe in diagnosis and treatment of tumor cells and living bodies. The diagnosis and treatment integrated nanoprobe can perform in-situ Raman diagnosis on tumor cells, is high in sensitivity and specificity, can realize photodynamic therapy at the same time, and has a certain application value in biological medicine and clinical diagnosis and treatment.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an integrated nanoprobe for diagnosis and treatment based on copper phthalocyanine molecules and its application. Background technique [0002] The integration of diagnosis and treatment is an emerging intelligent method in the medical field, a collaborative system with both diagnosis and treatment capabilities, and has become a highly concerned keyword in cancer research. Ideally, smart and efficient diagnostic-therapeutic probes should meet the following criteria: (i) monitoring and treatment modalities are activated specifically at the disease site to avoid background interference and side effects; in particular, activation of the probe with endogenous cancer markers Needles are ideal to provide precise control; (ii) probes should have high detection sensitivity and therapeutic efficacy; (iii) monitoring and treatment modalities need to be correlated in each probe to provide high pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/54A61K47/69A61K49/00A61P35/00B82Y5/00B82Y20/00
CPCA61K41/0076A61K47/6923A61K47/549A61K49/0013A61K49/0093A61P35/00B82Y20/00B82Y5/00
Inventor 田阳刘嘉郑婷婷
Owner EAST CHINA NORMAL UNIV
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