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Porcine Japanese encephalitis virus-specific positive serum and preparation method thereof

A technique for porcine Japanese encephalitis and positive serum, applied in chemical instruments and methods, antiviral immunoglobulin, immunoglobulin from serum, etc., can solve problems such as pollution and low serum titer

Active Publication Date: 2021-09-14
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sera prepared by the methods in the prior art are generally of low titer, and when pigs are used to prepare positive sera, it may also cause contamination of homologous other foreign viruses because they belong to homologous animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The preparation method of embodiment 1 porcine Japanese encephalitis virus specific positive serum

[0055] (1) Preparation of porcine Japanese encephalitis virus antigen.

[0056]The Japanese encephalitis SA14-14-2 strain was inoculated with Vero cells, which grew in a dense monolayer, at 37°C, 5% CO 2 The culture is carried out under the conditions, and the venom is harvested when 50-75% of the cells have pathological changes, and used as the porcine Japanese encephalitis virus antigen in the basic immunization. Store at -70°C.

[0057] (2) Preparation of porcine Japanese encephalitis virus inactivated vaccine.

[0058] 1) Selection of adjuvant: The polymer combination adjuvant of the animal vaccine used consists of water for injection, polymer, emulsifier, and auxiliary agent. Add the auxiliary agent, emulsifier, and polymer combination in the following proportions to the aqueous solution for injection in turn, stir while adding, and control the stirring speed at ...

Embodiment 2

[0077] The preparation method of embodiment 2 porcine Japanese encephalitis virus specific positive serum

[0078] (1) Preparation of porcine Japanese encephalitis virus antigen.

[0079] The Japanese encephalitis SA14-14-2 strain was inoculated with Vero cells, which grew in a dense monolayer, at 37°C, 5% CO 2 The culture is carried out under the conditions, and the venom is harvested when 50-75% of the cells have pathological changes, and used as the porcine Japanese encephalitis virus antigen in the basic immunization. Store at -70°C.

[0080] (2) Preparation of porcine Japanese encephalitis virus inactivated vaccine.

[0081] 1) Selection of adjuvant: The polymer combination adjuvant of the animal vaccine used consists of water for injection, polymer, emulsifier, and auxiliary agent. Add the auxiliary agent, emulsifier, and polymer combination in the following proportions to the aqueous solution for injection in turn, stir while adding, and control the stirring speed at...

Embodiment 3

[0099] The preparation method of embodiment 3 porcine Japanese encephalitis virus specific positive serum

[0100] (1) Preparation of porcine Japanese encephalitis virus antigen.

[0101] The Japanese encephalitis SA14-14-2 strain was inoculated with Vero cells, which grew in a dense monolayer, at 37°C, 5% CO 2 The culture is carried out under the conditions, and the venom is harvested when 50-75% of the cells have pathological changes, and used as the porcine Japanese encephalitis virus antigen in the basic immunization. Store at -70°C.

[0102] (2) Preparation of porcine Japanese encephalitis virus inactivated vaccine.

[0103] 1) Selection of adjuvant: The polymer combination adjuvant of the animal vaccine used consists of water for injection, polymer, emulsifier, and auxiliary agent. Add the auxiliary agent, emulsifier, and polymer combination in the following proportions to the aqueous solution for injection in turn, stir while adding, and control the stirring speed at...

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Abstract

The invention relates to a preparation method of serum, in particular to a porcine Japanese encephalitis virus-specific positive serum and a preparation method thereof. In the preparation method, guinea pigs or rabbits are used as immunized animals, and the virus liquid of passaged cells is used as porcine Japanese encephalitis virus antigen for basic immunization. The obtained Japanese encephalitis-specific positive serum titer is as high as 1:2000 times, which is higher than that obtained from directly immunized pigs, and can meet the requirements of Japanese encephalitis diagnosis technology and vaccine quality control. At the same time, the immunized animals are rabbits and guinea pigs, which reduces the cost and reduces the risk of homologous foreign virus contamination caused by homologous animals in preparing positive serum from pigs.

Description

technical field [0001] The invention relates to a preparation method of serum, in particular to a porcine Japanese encephalitis virus-specific positive serum and a preparation method thereof. Background technique [0002] Japanese encephalitis (JE) is a zoonotic disease transmitted by mosquitoes caused by Japanese encephalitis virus (JEV), and it is an acute disease of the central nervous system that seriously threatens the health of humans and animals. infectious disease. Pig is an important storage host and amplification host of Japanese encephalitis virus, the main source of infection of Japanese encephalitis, and one of the main pathogens that cause reproductive disorders in sows. Vaccination of pigs before the epidemic season of Japanese encephalitis can effectively reduce the incidence of human Japanese encephalitis. At present, there is still no specific drug to treat Japanese encephalitis infection, and it is mainly prevented and controlled through vaccination meas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K16/06
CPCC07K16/065C07K16/1081C07K2317/14C07K2317/35
Inventor 韩伟周建民于雷宗树成潘京学
Owner CHINA ANIMAL HUSBANDRY IND
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