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Application of hermetia illucens in transformation of microcystis mud and degradation of algal toxins

A technology of bright-spotted flat-horned soldier flies and cyanobacteria, which is applied in the field of biotechnology, can solve the problems of cyanobacteria and algae mud pollution, and achieve the effect of low cost and short conversion cycle

Inactive Publication Date: 2020-04-17
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides the application of the light-spotted flathorned soldier fly in transforming cyanobacteria mud while degrading algae toxins. The method provided by the invention can not only solve the pollution problem of cyanobacteria mud flooding, but also has low cost and can obtain insects with high added value. protein, but also can degrade algae toxins in cyanobacteria mud, solve the pollution of cyanobacteria mud to the environment and the impact on animal and human health

Method used

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  • Application of hermetia illucens in transformation of microcystis mud and degradation of algal toxins
  • Application of hermetia illucens in transformation of microcystis mud and degradation of algal toxins
  • Application of hermetia illucens in transformation of microcystis mud and degradation of algal toxins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The application of light-spotted soldier fly in the transformation of cyanobacteria mud and the degradation of algal toxins

[0029] ① Use toxic algae powder (the dominant species is Microcystisaeuginosa) to mix with bran at a ratio of 10% of dry weight, then add water to adjust the moisture content to 70%. The specific formula is shown in the following table:

[0030] Table 1 Soldier flies transform the formula of blue-green algae mud

[0031]

[0032] ② After adding the mixed feed to each bucket, add 400 soldier fly larvae (8 days old) and allow them to feed freely, with 4 repetitions in each group. Control the room temperature at 28±2°C and the ambient humidity at 40%-60%.

[0033] ③ Sampling. Get the original material, the original larvae, and then get the transformed material and soldier fly larvae on the 10th day.

[0034] ④ The material reduction rate, soldier fly conversion rate, and larval mortality of each group were counted separately.

[0035] ⑤ Measu...

Embodiment 2

[0051] Application of the larvae of the light-spotted soldier fly in the transformation of cyanobacteria mud:

[0052] Proceed as follows:

[0053] ① Spirulina algae mud (non-toxic cyanobacteria, mainly Microcystisflos-aquae) has a moisture content of 96.3%, and the quantitative bran amount is 100g. Add different amounts of cyanobacteria and algae mud, and adjust the moisture content at 65-80% at the same time Between, the ratio is set as follows:

[0054] Table 3 Soldier fly larva transformation formula composition of blue-green algae mud and bran compound

[0055]

[0056]

[0057] Note: The amount of bran and cyanobacteria mud in the table are all fresh weight

[0058] ② After adding the mixed feed to each bucket, add 200 soldier fly larvae (8 days old) and allow them to eat freely, with 4 repetitions in each group. Control the room temperature at 28±2°C and the ambient humidity at 40%-60%.

[0059] ③ After 50% of the prepupa appear, the insect material is separated...

Embodiment 3

[0069] Application of the larvae of the light-spotted soldier fly in the transformation of cyanobacteria mud:

[0070] Proceed as follows:

[0071] ①Cyanobacteria algae mud (mainly Microcystisflos-aquae) has a moisture content of 95.0%, and the amount of quantitative corn husks (moisture content 11%) is 100g. The moisture content is 52%), and finally adjust the moisture content to about 70%, and the ratio is set as follows:

[0072] Table 5 Soldier fly larva transforms the formula composition of blue-green algae mud and corn bran compound

[0073]

[0074] Note: The amount of bran and cyanobacteria mud in the table are all fresh weight

[0075] ② After adding the mixed feed to each bucket, add 400 soldier fly larvae (8 days old) and allow them to feed freely, with 4 repetitions in each group. Control the room temperature at 28±2°C and the ambient humidity at 40%-60%.

[0076] ③ After 50% of the prepupa appear, the insect material is separated.

[0077] ④ The 50% prepup...

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Abstract

The invention belongs to the field of biological science and technology, and specifically discloses an application of hermetia illucens in transformation of microcystis mud and degradation of algal toxins. Applicants transform the microcystis mud containing the algae toxins by using the hermetia illucens and find that the hermetia illucens has a certain degradation effect on the algal toxins in the microcystis mud, wherein the degradation rate is 40.98%, after the microcystis mud is transferred by the hermetia illucens, only 0.81% of the algal toxins are transferred into hermetia illucens bodies, and the transfer amount is small; bran, corn bran and the microcystis mud are mixed according to a certain ratio to feed larvae of the hermetia illucens to transform the microcystis mud, and a better transformation effect can be obtained; and a transformation method provided by the invention can effectively transform the microcystis mud to obtain hermetia illucens larvae protein, the transformation cycle takes only 12-13 days, and the method can also degrade the algae toxins in the microcystis mud, and solve pollution of the microcystis mud to the environment and influence of the microcystis mud on health of animals and humans.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of light-spotted flathorned soldier flies in transforming cyanobacteria mud while degrading algae toxins. The invention can not only solve the pollution problem of cyanobacteria mud flooding in lakes, but also has low cost and can obtain High value-added insect protein can reduce the environmental pollution caused by cyanobacteria algae mud and algae toxins. Background technique [0002] Summer and autumn are the periods of high cyanobacteria blooms in eutrophic water bodies. Some lakes and reservoirs have to harvest cyanobacteria to form a large amount of algae mud, some of which contain algae toxins, and their content can reach 1 / 1000 of the dry weight. Algal toxins have cancer-promoting effects, liver toxicity, nephrotoxicity, enterotoxicity, and other toxicities. The existing cyanobacteria algae mud treatment technology, such as anaerobic fermentation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/37A23K10/16C05F15/00C05F17/05
CPCA01K67/033A23K10/16A23K10/37A23K50/90C05F5/00C05F5/002Y02P60/87Y02W30/40
Inventor 张吉斌汪冕王高鸿蔡珉敏郑龙玉李林霍岩宋立荣杨洋喻子牛
Owner HUAZHONG AGRI UNIV
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