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Amino acid metabolomics-based method for researching anti-insomnia action mechanism of sugemule

A metabolomics and mechanism of action technology, applied in the field of amino acid metabolomics to study the anti-insomnia mechanism of Sugermuller, which can solve the problems of column adsorption, low polar volatility, etc., and achieve the effect of optimizing the measurement conditions.

Active Publication Date: 2020-04-14
田继兰
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Problems solved by technology

Metabolites in living organisms, such as amino acids, fatty acids, amines, sugars, and nucleotides, have the common characteristics of strong polarity and low volatility, and are easily adsorbed by chromatographic columns, so they are not suitable for direct GC-MS analysis

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  • Amino acid metabolomics-based method for researching anti-insomnia action mechanism of sugemule
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  • Amino acid metabolomics-based method for researching anti-insomnia action mechanism of sugemule

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Embodiment Construction

[0039] The present invention will be further described with reference to the accompanying drawings and embodiments.

[0040] In one embodiment, as figure 1 As shown, the described method based on amino acid metabolomics to study the mechanism of anti-insomnia action of Sugermuller:

[0041] 1. Making PCPA to insomnia rat model

[0042] The rats were randomly divided into normal control group, model control group, Mongolian medicine group and Anshen Bunao oral liquid group according to their body weight, with 10 rats in each group; except for the normal control group, the other three groups of rats were given intraperitoneal injection at 8:00 am every day. Injection of PCPA suspension 1 ml / 100g, once a day, for 2 consecutive days, the normal control group was intraperitoneally injected with the same volume of weak alkaline saline; 24-28h after the first intraperitoneal injection, the animals appeared circadian rhythm disappearance , and kept active during the day, indicating ...

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Abstract

The invention relates to an amino acid metabolomics-based method for researching an anti-insomnia action mechanism of sugemule. Amino acid metabolome in each of tissues of the brains of para-chlorophenylalanine (PCPA) insomnic model rats before and after gavage with the sugemule is analyzed by applying a gas chromatography-mass spectrometer (GC-MS) method; endogenous compounds are identified by astandard fingerprint bank; changes of metabolite maps of all parts of the brains are analyzed and related metabolic markers are assayed by employing principal component analysis (PCA) and partial least squares-discrimination analysis (PLS-DA) modes; a targeting metabolomics research is conducted by applying liquid chromatograph-mass spectrometer (LC-MS) to explore content changes of neurotransmitters including 5-hydroxytryptamine, gamma-aminobutyric acid (GABA), glutamic acid (Glu) and the like in the brain tissues and related metabolites; by seeking for difference metabolites related with thecurative effect of the sugemule and metabolic pathways of the difference metabolites from brain tissue samples of clinical rats, the anti-insomnia action mechanism and an action site of the sugemuleare specified; and a metabolomics research means is applied for the first time by the amino acid metabolomics-based method to preliminarily confirm the action mechanism of the sugemule regulating andcontrolling a sleep-wake rhythm by a neurotransmitter system.

Description

technical field [0001] The invention belongs to the cross technical field of traditional Chinese medicine technology and metabolomics technology, and specifically relates to a method for studying the anti-insomnia action mechanism of Sugermuller based on amino acid metabolomics. Background technique [0002] Sleep accounts for about one-third of a normal person's daily time, and is an important physiological process to maintain the normal life activities of the human body. In recent years, with the rapid economic development, the rhythm of life has been accelerated, occupational and environmental pressures have become more and more serious, the incidence of insomnia has also continued to rise, and the trend of younger people has become more and more obvious, which has seriously affected people's normal life. work, life and health. Insomnia has become a common and frequently-occurring disease in neurology outpatient clinics, with clinical manifestations such as difficulty fa...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K49/00A61K36/9064A61P25/20
CPCA01K67/02A61K49/0008A61K36/9064A61K36/28A61K36/67A61K36/725A61P25/20A61K2300/00
Inventor 田继兰
Owner 田继兰
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