Ultrasonic-assisted Ganoderma lucidum polysaccharides extraction and acid hydrolysis and monosaccharide composition detection methods

Abstract

The invention, which belongs to the technical field of polysaccharide extraction and detection, particularly relates to ultrasonic-assisted Ganoderma lucidum polysaccharides extraction and acid hydrolysis and monosaccharide composition detection methods. The method for ultrasonic-assisted Ganoderma lucidum polysaccharides extraction and acid hydrolysis has the remarkable advantages of high speed,high efficiency and the like, and rapid analysis and determination of the monosaccharide composition of Ganoderma lucidum polysaccharides in combination with a UPLC-CAD method. The monosaccharide composition and content of the Ganoderma lucidum polysaccharides acid hydrolysis product are compared and analyzed. The results show that the content of xylose in the complete hydrolysate of Ganoderma lucidum polysaccharides is the highest, arabinose has the second content, the contents of rhamnose, galactose and fucose are close, the contents of glucose and mannose are low; and research results provide the data support and technical reference for evaluating the quality of glossy ganoderma medicinal materials and guiding further development and utilization.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction and monosaccharide composition detection, in particular to an ultrasonic-assisted extraction and acid hydrolysis of Ganoderma lucidum polysaccharide and a detection method for determining monosaccharide composition in Ganoderma lucidum polysaccharide by an ultra-high liquid chromatography-electric aerosol detector. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Ganoderma Lucidum polysaccharides (Ganoderma Lucidum polysaccharides) is the secondary metabolite of Ganoderma lucidum mycelium and the main functional component of Ganoderma lucidum. Polysaccharides are formed by com...

AI Technical Summary

Problems solved by technology

But at the same time, it is also found that the traditional high-temperature acid hydrolysis method is used for the hydrolysis of polysaccharides, which takes a long time, consumes a lot of energy, and has low efficiency.

Ultrasonic extraction technology is to use the strong cavitation effect and mechanical vibration generated by ultrasonic waves to accelerate the active ingredients of drugs into the solvent, promote the extraction, increase the dissolution rate of active ingredients, improve the utilization of medicinal materials, save energy and avoid high temperature damage to the extracted ingredients. It has the advantages of saving time, energy, and high extraction rate. It has been widely used in the extraction of active ingredients in natural products, but there are few reports on the application of polysaccharide hydrolysis.

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