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Method for detecting comprehensive content of soil heavy metal by luminous bacteria and application thereof

A technology of luminescent bacteria and heavy metals, applied in measuring devices, chemiluminescence/bioluminescence, and analysis through chemical reactions of materials, can solve problems that have not yet existed, and achieve fast reproduction speed, strong vitality, and great application prospects Effect

Pending Publication Date: 2020-04-10
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no method to extend the luminescent bacteria from water pollution detection to soil pollution detection, and to detect whether the discharge of industrial wastewater is up to standard and cause heavy metal pollution in the soil. There is also no effective and rapid method for comprehensive toxicity detection of heavy metals and quantitative description of soil. Method for comprehensive content of heavy metal ions

Method used

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  • Method for detecting comprehensive content of soil heavy metal by luminous bacteria and application thereof
  • Method for detecting comprehensive content of soil heavy metal by luminous bacteria and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for photobacteria detection of soil heavy metal comprehensive content of the present embodiment comprises the following steps:

[0026] 1) Sample pretreatment:

[0027] Collect the soil near a plastic factory and remove debris, including gravel, stones, sticks, weeds, plant roots, insect corpses, stones, and new bodies such as manganese nodules, lime nodules, etc.;

[0028] Spread the soil after removing debris on a drying board or wooden board lined with clean white paper to dry naturally, and exposure to the sun is strictly prohibited. When the soil is semi-dry, break up large pieces of soil to avoid forming hard lumps, and pick up gravel, animal and plant residues at any time during the air-drying process. The air-drying room ensures drying and ventilation, the air-drying temperature is 30-35°C, and the air-drying time is 3-7 days;

[0029] Grind the air-dried soil with a grinding rod. First, it needs to pass through a nylon sieve with a pore size of 2mm, ...

Embodiment 2

[0046] The difference between embodiment 2 and embodiment 1 is:

[0047] In this embodiment, the soil near a certain discontinued factory was collected as a test sample. After removing gravel, stones, weeds, and plant residues, the sample was ground, and then ground and sieved 3 times after passing through a 2mm sieve until only A small amount of sand is stopped, and then passed through a 100-mesh fine sieve. The sieved soil was made into a soil suspension at a ratio of soil: water = 1:5, shaken on a shaking table for 8 hours, and after standing for 16 hours, the supernatant was obtained, filtered with a 20-micron ultrafiltration membrane, and the filtrate was filtered at a speed of Centrifuge at 7000 rpm for 3 minutes to obtain a colorless and transparent solution as the sample to be tested.

[0048] In this example, the relative luminous intensity of the obtained sample is 0.773%, corresponding to the toxicity interval II level, and the total toxicity of the soil is equival...

Embodiment 3

[0050] The difference between embodiment 3 and embodiment 1 is:

[0051] In this embodiment, the soil near the metal processing plant is collected as a test sample, and the sample is ground after removing gravel, stones, weeds, and plant residues, and then ground after passing through a 2mm sieve, and sieved 5 times until only a small amount The sand particles are stopped, and then passed through a 100-mesh fine sieve. The sieved soil was made into a soil suspension at a ratio of soil: water = 1:5, shaken on a shaking table for 8 hours, and after standing for 16 hours, the supernatant was obtained, filtered with a 20-micron ultrafiltration membrane, and the filtrate was filtered at a speed of Centrifuge at 9000 rpm for 3 minutes to obtain a colorless and transparent solution as the sample to be tested.

[0052] In this embodiment, the relative luminous intensity of the obtained sample is 0.553%, corresponding to the toxicity interval II level, and the total toxicity of the so...

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Abstract

The invention, which belongs to the field of environmental detection by a biological method, discloses a method for detecting the comprehensive content of heavy metal in soil by luminous bacteria andapplication thereof. The method comprises the following steps: removing impurities from a collected soil sample, carrying out air drying, grinding and sieving, adding water to prepare a soil suspension, and carrying out oscillation, standing, filtration and centrifugation to obtain a to-be-detected sample; and determining a relation between the relative luminous intensity of the vibrio fischeri and the concentration of the heavy metal ions by using the vibrio fischeri with low cost in luminous bacteria and establishing a mathematical model to enable each relative luminous intensity to have a heavy metal ion toxicity interval corresponding to the relative luminous intensity, thereby conveniently and quickly detecting the heavy metal pollution of the soil. The method is simple to operate, single in equipment, low in cost and wide in applicability, and has important significance in soil pollution detection.

Description

technical field [0001] The invention belongs to the field of environmental detection by biological methods, and in particular relates to a method for detecting the comprehensive content of soil heavy metals by luminescent bacteria and an application thereof. Background technique [0002] Soil is a very important part of nature and plays an indispensable role in maintaining ecological balance and energy cycle. Urban soil is an important factor affecting the urban environment, and it also seriously affects human health. Domestic sewage and industrial wastewater are the main sources of urban soil pollution; among them, heavy metal ions such as copper, nickel, zinc, lead, and cadmium will damage the ecological environment in the soil, lead to ecological imbalance, and endanger human society and human health. For example, nickel, a heavy metal ion, can cause lung cancer, nose cancer, throat cancer and prostate cancer; studies have shown that cadmium can cause kidney and lung dis...

Claims

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Application Information

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IPC IPC(8): G01N21/76
CPCG01N21/763
Inventor 江伟王昆肖昕吴晋芝陆方筱张良
Owner HUAQIAO UNIVERSITY
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