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Artificial seawater composition, Shewanella loihica cultured by same and application of thereof

A technology of artificial seawater and Shewanella, applied in the direction of application, microbial-based methods, biochemical equipment and methods, etc., can solve the problem of low growth ability and achieve good breeding effects

Pending Publication Date: 2020-04-10
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, previous experiments have found that the growth ability of artificial seawater cultured amphioxus and prawns is significantly lower than that of natural seawater cultured, and there are no reports on using artificial seawater instead of natural seawater to cultivate marine bacterial biofilms and induce spat to attach

Method used

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  • Artificial seawater composition, Shewanella loihica cultured by same and application of thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Artificial Seawater Composition Cultivation of Marine Shewanella

[0014] The single colony obtained by coating and streaking the pure strain of Marine Shewanella in 2216E solid medium is transferred to Zobell 2216E liquid medium for expansion, and then centrifuged and washed to make a suspension. Then stain with acridine orange and randomly select 10 points to count under the 1000 times lens of a fluorescence microscope to determine that the total density of the bacterial solution is 2.1×10 9 cells / mL.

Embodiment 2

[0015] Example 2 Induction experiment of marine Shewanella on the attachment of juvenile mussels

[0016] The initial bacterial liquid density in Example 1 was selected to be 1×10 6 cells / mL, 1×10 7 cells / Ml, 1×10 8 cells / mL and 5×10 8 Cells / mL of marine Shewanella liquid, put a sterilized glass slide in a sterilized petri dish, and add sterilized natural seawater and the artificial seawater configured in the present invention to make the total volume of liquid in the petri dish It is 20mL, cultured for 48h at 18°C ​​in the dark to form a biofilm. Transfer a glass slide with a biofilm to a new petri dish containing sterilized natural seawater, and evenly put 10 thick-shelled juvenile mussels (shell length 0.68±0.04mm, shell Height 0.46±0.02mm), record the attachment rate of juveniles at 24h, that is, the percentage of juveniles attached to the glass slide to the total number of juveniles in the petri dish, the result is as follows figure 1 Shown.

[0017] by figure 1 It can be s...

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Abstract

The invention discloses an artificial seawater composition, Shewanella loihica cultured by the artificial seawater composition and an application of the Shewanella loihica. The composition is calculated by 1000mL of distilled water, and also contains 28 g of sodium chloride; 7g of magnesium sulfate heptahydrate, 4g of magnesium chloride hexahydrate, 0.70 g of potassium chloride, 1.11 g of calciumchloride, 0.10 g of sodium nitrate, 0.01 g of potassium phosphate, 0.014 g of beta-disodium glycerophosphate pentahydrate; 0.15 g of sodium silicate nonahydrate, 0.10 mL of vitamin B12 with a concentration of 2 [mu] g / mL, 1 mL of vitamin B7 with the concentration of 1 [mu] g / mL, 1 mL of vitamin B1 with the concentration of 100 [mu] g / mL, 10 mL of pIIMetals, 10 mL of S2Metals, 1 g of Tris and 0.10g of nitrilotriacetic acid. The effect of inducing juvenile mytilus coruscus to be attached by a biofilm formed by Shewanella loihica cultured by a culture medium is similar to that of natural seawater, and the culture medium has a guiding effect on culture and breeding of mytilus coruscus.

Description

Technical field [0001] The present invention relates to a formulation of an artificial seawater composition, in particular to an artificial seawater composition used to replace natural seawater to cultivate marine bacteria, especially for cultivating marine Shewanella loihica, and verifying the marine Shewanella loihica The effect of biofilm on the adhesion of juvenile thick-shelled mussels. Background technique [0002] Thick-shelled mussel (Mytilus coruscus), belonging to Mollusca (Mollusca), Bivalvia (Bivalvia), Mytilodia (Mytilodia), Mytilidae (Mytilidae), distributed in the Yellow Sea, Bohai Sea and East China Sea coast, is my country An important species of marine shellfish culture. Thick-shelled mussels develop from fertilized eggs to eye-point larvae, and then through the process of attachment and metamorphosis, they develop into juveniles, and finally into adult shells. During the growth and development process from juveniles to adults, when the environment changes, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01K61/54C12R1/01
CPCC12N1/20A01K61/54Y02A40/81
Inventor 梁萧常睿珩彭莉华杨金龙
Owner SHANGHAI OCEAN UNIV
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