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Bivalent inactivated vaccine for pigs, preparation method and application thereof

A dual inactivated vaccine and inactivation technology, applied in botany equipment and methods, biochemical equipment and methods, applications, etc.

Pending Publication Date: 2020-04-10
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no commercially available vaccine for Seneca virus

Method used

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  • Bivalent inactivated vaccine for pigs, preparation method and application thereof
  • Bivalent inactivated vaccine for pigs, preparation method and application thereof
  • Bivalent inactivated vaccine for pigs, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 pig uses the preparation of double inactivated vaccine

[0023] 1 poison seed

[0024] Porcine Seneca virus, its microbial preservation number is: CGMCC No.18851; classification name: Seneca virus; preservation unit: General Microbiology Center of China Committee for the Collection of Microorganisms; preservation time is October 29, 2019; preservation Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0025] 2 test method

[0026] 2.1 Preparation of porcine foot-and-mouth disease baculovirus

[0027] 2.1.1 Design and synthesis of FMDV-vp1 gene

[0028] The O-type FMDV VP1 gene (SEQ ID NO.1) was codon-optimized to obtain the optimized sequence (SEQ ID NO.2), and EcoRI and Hind III restriction sites were added to both ends of the optimized sequence and cloned into the pFastBacⅠ vector , forming heavy pFB-FMDV-vp1.

[0029] 2.1.2 Construction and preparation of recombinant shuttle bacmid rBacmid-FMDV-vp1

[0030] 2.1.2.1 Constru...

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Abstract

The invention discloses a bivalent inactivated vaccine for pigs, a preparation method and application thereof. The bivalent inactivated vaccine for pigs disclosed by the invention comprises: a recombinant baculovirus for expressing an O-type FMDV VP1 gene, a porcine senecavirus and an immunoadjuvant. The invention further discloses a preparation method of the bivalent inactivated vaccine for pigs,and the method includes: (1) amplifying the recombinant baculovirus expressing the O-type FMDVVP1 gene, and then performing inactivation, amplifying the porcine senecavirus, and then performing inactivation; (2) mixing the inactivated recombinant baculovirus liquid expressing the O-type FMDV VP1 gene with the inactivated porcine senecavirus liquid uniformly to obtain a water phase; (3) heating the immunoadjuvant to obtain an oil phase; and (4) adding the oil phase into the water phase, and performing mixing and emulsification. Immune protection efficacy detection results prove that the bivalent inactivated vaccine for pigs prepared according to the invention can effectively control O-type foot-and-mouth disease virus and porcine senecavirus at the same time.

Description

technical field [0001] The invention relates to a dual inactivated vaccine for pigs, in particular to porcine foot-and-mouth disease baculovirus carrier, porcine Seneca virus dual inactivated vaccine and a preparation method thereof, belonging to the field of dual inactivated vaccines for pigs and their application. Background technique [0002] Type O FMDV is a member of the genus FMDV and the family Picornaviridae. Among all seven serotypes of foot-and-mouth disease virus, type O is the most prevalent and widely distributed in the world, and has been reported in northern Africa, Eastern Europe, the entire South America and most parts of Asia, seriously threatening the development of global animal husbandry. In the preparation process of inactivated vaccines, due to the disadvantages of high equipment safety requirements, complicated process, high cost, incomplete inactivation of virus strains, and the risk of loose virus, baculovirus vector vaccines are considered to be th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/135A61K39/12A61K39/39A61P31/14C12N15/867
CPCA61K39/12A61K39/39A61P31/14C12N15/86C12N2770/32034C12N2770/32134C12N2710/14043C12N2800/107A61K2039/70A61K2039/53A61K2039/5156A61K2039/55566
Inventor 宋扬柴华杨淑萍刘鑫莹李应鹤武啸李贽郭照成马萌萌孟福强
Owner 哈药集团生物疫苗有限公司
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