Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium for saprophiticnematode in infected wood of pine wood nematodes and preparation method and application of culture medium

A technology for pine wood nematodes and culture medium, which is applied in animal husbandry and other directions, can solve the problems of complex nematode culture medium composition, long saprophytic nematode culture time, and difficulty in obtaining raw materials, etc., so as to shorten the culture time, accelerate the speed, and easily obtain the price. Effect

Active Publication Date: 2020-04-10
NANJING FORESTRY UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the deficiencies in the prior art, the technical problem to be solved by the present invention is to provide a culture medium for saprophytic nematodes of pine wood nematode disease, so as to solve the problems in the prior art that the components of the nematode culture medium are complex, the required raw materials are difficult to obtain, and the price is expensive; The technical problem to be solved by the present invention is to provide the preparation method of the culture medium of saprophytic nematode of pine wood nematode disease, to solve the complex problem of configuration program in the prior art; Application in saprophytic nematodes of wood nematodes infested wood to solve the problem of long culture time of saprophytic nematodes in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium for saprophiticnematode in infected wood of pine wood nematodes and preparation method and application of culture medium
  • Culture medium for saprophiticnematode in infected wood of pine wood nematodes and preparation method and application of culture medium
  • Culture medium for saprophiticnematode in infected wood of pine wood nematodes and preparation method and application of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Saprophytic nematode basal medium screening and determination of main components

[0026] 1) Effects of different media on the growth of nematodes

[0027] 25 nematodes were inoculated into NGM-sawdust medium and PDA-sawdust medium plates respectively, sealed with parafilm, and NGM and PDA without sawdust were used as controls, placed in an incubator at 25°C for 14 days, and calculated The number of nematodes in each plate.

[0028] NGM-sawdust medium: 3g sodium chloride, 17g agar powder, 2.4g tryptone, 20g sawdust, add water to 1000mL, after autoclaving, cool the culture medium to 60°C, add 1mol CaCl under sterile conditions 2 1mL, 1mol MgSO 4 1mL, 1mL of 5mg / mL cholesterol, 25mL of 1mol phosphate buffer (pH 6.0), mix quickly, make a plate, and store at room temperature.

[0029] PDA-sawdust medium: 200g potatoes, washed and peeled, cut into small pieces, add 1000mL of water and boil for 25min; filter through gauze, add water to the filtrate to make up 10...

Embodiment 2

[0045] The screening of embodiment 2 culture medium main effect factor

[0046] 1) The effect of glucose concentration on the growth of nematodes

[0047] The effect of glucose concentration on the growth of nematodes was investigated by single factor experiment. On the basis of the basic medium, change the concentration of glucose, that is, when the concentration of glucose is 10, 15, 20, 25, 30g / L, inoculate 25 saprotrophic nematodes, and calculate the number of nematodes in each plate after culturing at 25°C for 14 days .

[0048] Depend on figure 1 It can be seen that with the change of glucose concentration, the growth of nematodes also changed. When the glucose concentration was 20g / L and 25g / L, the number of nematodes was significantly different from other treatments (P0.05).

[0049] 2) The effect of sawdust content on nematode growth

[0050] On the basis of basal medium, the effect of sawdust content on nematode growth at 10, 20, and 30 g / L was investigated. In...

Embodiment 3

[0055] Embodiment 3 optimizes medium proportioning

[0056] Glucose, sawdust and agar were selected as three medium components, and an orthogonal experiment with three factors and three levels was designed to optimize the proportion of the medium.

[0057] It can be seen from Table 3 of the orthogonal test that the test results of group 8 are the best, so 30g / L of sawdust, 20g / L of glucose, and 30g / L of agar are the optimal level combination of the test.

[0058] Compute the sum of replicate indices at each level, with K 1 、K 2 、K 3 said, and then by comparing K 1 、K 2 、K 3 Corresponding to the estimated value of the level effect of each column of factors, it can be concluded that the theoretical optimal combination ratio of saprotrophic nematode medium is: sawdust 30g / L, glucose 10g / L, agar 30g / L.

[0059] Table 3 Orthogonal test table

[0060]

[0061] 25 saprotrophic nematodes were cultured with the medium ratio of the experimental optimal combination and the theo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a culture medium for saprophiticnematode in infected wood of pine wood nematodes and a preparation method and application of the culture medium and belongs to the technical field of culture of saprophiticnematode. The formula of the saprophiticnematode culture medium comprises 20-30g / L of pine wood bits, 10-20g / L of glucose and 20-30g / L of agar. After high-pressure steam sterilization, cooling and curing, the culture medium is inoculated with saprophiticnematode, the saprophiticnematode is cultured for 10 days or longer in an incubator at 25 DEG C, and the saprophiticnematode is collected, wherein the pine wood bits are wood bits of xylem of an original pinus tree variety from which the saprophiticnematode can be separated. Compared with a PDA (potato dextrose agar)culture medium which is full of botrytis cinerea for culturing the saprophiticnematode and takes 22-30 days to obtain a great deal of saprophiticnematode, the culture medium is capable of shorteningthe culture time by a half or greater, increasing the speed of indoor culture of the saprophiticnematode and improving the culture efficiency; and in addition, the culture medium is simple in preparation step, expected raw materials are easy to obtain and low in price, and large-scale production and use can be easily achieved.

Description

technical field [0001] The invention belongs to the technical field of saprophytic nematode culture, and in particular relates to a culture medium for saprophytic nematodes of pine wood nematode disease and its preparation method and application. Background technique [0002] Saprophytic nematodes refer to the nematodes that feed on sugar and protein decomposition products of bacterial action (Liu Nanxin et al., 1982), or feed on decayed organic debris. Saprotrophic nematodes are present in large numbers in the soil and are often seen in and around plant roots and dead and decaying tissue in the underground or aerial parts of plants. Saprophytic nematodes in diseased and dead wood of pine wood nematode are also extremely common (Ren-E et al, 2010; Li Xiangkang et al, 2010; Wang Xinrong et al, 2008), but people have little knowledge about the morphology, physiology and ecology of saprophytic nematodes in dead wood of pine wood nematode. Research is still limited, and one of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 叶建仁史丽娜
Owner NANJING FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com