Method and culture medium for efficiently culturing dendrobium huoshanense seedlings
A culture medium, Dendrobium officinale technology, which is applied in the field of seedling propagation of endangered and rare medicinal plants, can solve the problems of loose control of hormone components, low survival rate of transplanting, lack of resources, etc. The effect of strong operability and easy availability of materials
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Embodiment 1
[0025] Embodiment 1: a kind of method of high-efficiency cultivation rice dendrobium seedling, comprises the following steps:
[0026] (1) Sterile sowing: take the mature and uncracked rice dendrobium capsules as explants, disinfect with 72% (V / V) alcohol for 50 seconds, rinse twice with sterile water, and then use 1% Disinfect with sodium hypochlorite for 10 minutes, then rinse with sterile water for 5 times and then dry; cut the fruit, use an inoculation needle to inoculate the yellow powdery seeds into a culture bottle containing about 100ml of seed germination medium for cultivation, and the seeds will germinate into protocorms , the seed germination medium is 1 / 2N6 medium+6-BA 1.0mg / L+NAA0.3mg / L+150g / L banana+25g / L sucrose+5g / L agar+0.8g / L activated carbon, The rest is water; a plump dark green and dense protocorm with a diameter of about 1.5-2.0 mm is obtained.
[0027] (2) Protocorm differentiation: Inoculate dense protocorms obtained by aseptic sowing into a culture b...
Embodiment 2
[0032] Embodiment 2 A method for efficiently cultivating rice seedlings of Dendrobium, comprising the following steps:
[0033] (1) Sterile sowing: take the mature and uncracked rice dendrobium capsules as explants, disinfect with 72% (V / V) alcohol for 50 seconds, rinse twice with sterile water, and then use 1% Disinfect with sodium hypochlorite for 10 minutes, then rinse with sterile water for 5 times and then dry; cut the fruit, use an inoculation needle to inoculate the yellow powdery seeds into a culture bottle containing about 100ml of seed germination medium for cultivation, and the seeds will germinate into protocorms , the seed germination medium is 1 / 2N6 medium+6-BA 1.0mg / L+NAA0.3mg / L+150g / L banana+25g / L sucrose+5g / L agar+0.8g / L activated carbon, The rest is water; a plump dark green and dense protocorm with a diameter of about 1.5-2.0 mm is obtained.
[0034] (2) Protocorm differentiation: Inoculate dense protocorms obtained by aseptic sowing into a culture bottle f...
Embodiment 3
[0038] Embodiment 3: a kind of method for efficiently cultivating rice seedlings of Dendrobium, comprises the following steps:
[0039] (1) Sterile sowing: take the mature and uncracked rice dendrobium capsules as explants, disinfect with 72% (V / V) alcohol for 50 seconds, rinse twice with sterile water, and then use 1% Disinfect with sodium hypochlorite for 10 minutes, then rinse with sterile water for 5 times and then dry; cut the fruit, use an inoculation needle to inoculate the yellow powdery seeds into a culture bottle containing about 100ml of seed germination medium for cultivation, and the seeds will germinate into protocorms , the seed germination medium is 1 / 2N6 medium+6-BA 1.0mg / L+NAA0.3mg / L+150g / L banana+25g / L sucrose+5g / L agar+0.8g / L activated carbon, The rest is water; a plump dark green and dense protocorm with a diameter of about 1.5-2.0 mm is obtained.
[0040] (2) Protocorm differentiation: Inoculate dense protocorms obtained by aseptic sowing into a culture ...
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