A kind of heat-resistant shiitake mushroom strain and its cultivation method
A technology of shiitake mushrooms and strains, which is applied in the fields of mushroom cultivation, cultivation, plant cultivation, etc., and can solve problems such as rot of mushroom sticks and death of mycelium
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[0046] Embodiment 1, the breeding of high temperature resistant shiitake mushroom strain - shiitake mushroom height 16 × buckle 46
[0047] The main cultivars of Lentinus edodes High Temperature No. 8 (referred to as High Temperature 8) and Lentinus edodes Kouxiang (referred to as Kouxiang) were selected as the parent strains for hybrid breeding of high temperature resistant Lentinus edodes hybrid strains. details as follows:
[0048] 1.1 Test strains
[0049] Parental strains: Lentinus edodes High Temperature No. 8 and Lentinus edodes Kouxiang, the parental fruiting bodies with a round shape and 7 minutes of maturity were selected to collect single spores.
[0050] 1.2 Isolation and identification of single spores
[0051]The parental fruiting bodies are suspended in a triangular flask filled with sterile water for 3-5 days, diluted and spread on a PDA plate, cultured at 25°C for 3-5 days, and a single germinated colony is picked for transfer culture, and observed under a m...
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