High temperature resistant Lentinula edodes strain and cultivation method thereof
A technology of shiitake mushrooms and high temperature, which is applied in mushroom cultivation, cultivation, plant cultivation, etc., and can solve problems such as rot of mushroom sticks and death of mycelia
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[0046] Example 1. Breeding of high temperature resistant lentinus edodes strain-lentinus edodes 16×kou 46
[0047] The main summer cultivars of Lentinus edodes No. 8 (referred to as Gaotian No. 8) and Lentinus edodes Kouxiang (referred to as Kouxiang) were selected as the parent strains for cross breeding of high-temperature resistant Lentinus edodes hybrid strains. details as follows:
[0048] 1.1 Test strains
[0049] Parental strains: Shiitake No. 8 and Shiitake Kouxiang, select the round shape, 7-point mature parent fruiting bodies to collect monospores.
[0050] 1.2 Isolation and identification of monospores
[0051] The parent fruit body is suspended in a triangular flask filled with sterile water for 3-5 days, diluted and spread on a PDA plate, cultured at 25°C for 3-5 days, picked out a single germinated colony and transferred to culture, and observed under a microscope for locks joint. If there is no lock-like association, it can be preliminarily considered to be a monocytic...
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