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Preparation method of medical surgical biological patch

A biological patch and surgical technology, which is applied in the field of preparation of medical surgical biological patches, can solve the problems of self-tissue ingrowth, etc., and achieve the effects of low immunogenicity, good biocompatibility, and excellent flexibility

Inactive Publication Date: 2020-04-07
成都奇璞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to meet the repair needs of these defective tissues or organs, many different types of "surgical patches" have been developed at home and abroad to supply clinical needs, but each material has its own problems such as biomechanical properties, biocompatibility, immune Rejection, can induce self-organization ingrowth and other problems

Method used

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  • Preparation method of medical surgical biological patch
  • Preparation method of medical surgical biological patch
  • Preparation method of medical surgical biological patch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Material collection and pretreatment: cut adult healthy animal bovine pericardium, immediately wash it in ultrapure water at about 4°C for 3 times, use surgical tools to remove surface fat and fibers, and avoid contact with pollutants as much as possible; cut to a certain size The texture is uniform without damaged parts, put into 10% NaCl solution and shake and wash for 1 hour, repeat 3 times, and then put into ultrapure water to wash 4 times.

[0035] (2) Degreasing: Add the above-mentioned degreased pericardial material into chloroform:methanol=1:1 mixture, degrease by shaking for 3 hours, repeat twice; dehydration with gradient alcohol is 98%, 60% and 20% alcohol respectively for 6 and 2 and wash 4 times with ultrapure water for 1 hr.

[0036] (3) Decellularization: flatten the above-mentioned degreased pericardial material and add ultrapure water (just cover the pericardium with ultrapure water), then put it in a -80°C refrigerator for quick freezing for 5 hour...

Embodiment 2

[0043] (1) Material collection and pretreatment: cut adult healthy animal bovine pericardium, immediately wash it in ultrapure water at about 4°C for 3 times, use surgical tools to remove surface fat and fibers, and avoid contact with pollutants as much as possible; cut to a certain size The texture is uniform without damaged parts, put into 4% NaCl solution and shake and wash for 1 hour, repeat 3 times, and put into ultrapure water to wash 4 times again.

[0044] (2) Degreasing: Add the above-mentioned degreased pericardial material into chloroform:ethanol=1:1 mixture, degrease by shaking for 4 hours, repeat 3 times; dehydration with gradient alcohol is 90%, 50% and 30% alcohol respectively for 6 and 2 and wash 4 times with ultrapure water for 1 hr.

[0045] (3) Decellularization: flatten the above-mentioned degreased pericardial material and add ultrapure water (just cover the pericardium with ultrapure water), then put it in a -80°C refrigerator for quick freezing for 5 hou...

Embodiment 3

[0052](1) Material collection and pretreatment: cut adult healthy animal bovine pericardium, immediately wash it in ultrapure water at about 4°C for 3 times, use surgical tools to remove surface fat and fibers, and avoid contact with pollutants as much as possible; cut to a certain size The texture is uniform without damaged parts, put into 4% NaCl solution and shake and wash for 1 hour, repeat 3 times, and then put into ultrapure water to wash 4 times.

[0053] (2) Degreasing: Add the above-mentioned degreased pericardial material into chloroform:ethanol=1:1 mixture, degrease by shaking for 4 hours, repeat 3 times; dehydration with gradient alcohol is 90%, 50% and 30% alcohol respectively for 6 and 2 and wash 4 times with ultrapure water for 1 hr.

[0054] (3) Decellularization: flatten the above-mentioned degreased pericardial material and add ultrapure water (just cover the pericardium with ultrapure water), then put it in a -80°C refrigerator for quick freezing for 5 hours...

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Abstract

The invention discloses a preparation method of a medical surgical biological patch. The preparation method comprises the following steps: extracting an animal-derived material for pretreatment; treating the pretreated animal-derived material with an organic solvent, conducting dehydrating with gradient alcohol, and performing degreasing; repeatedly freezing and thawing the degreased animal-derived material, and carrying out decellularization; soaking the decellularized animal-derived material in an acidic solution of an enzyme, and carrying out enzymatic digestion; subjecting the animal-derived material having undergone enzymatic digestion to alkali treatment in alkaline solutions with different concentrations; and descaling and sterilizing the animal-derived material having undergone alkali treatment to obtain the medical surgical patch. With the preparation method of the medical surgical biological patch provided by the invention, the obtained biological patch has excellent biomechanical properties and biocompatibility, has no immune exclusion and can induce growth of autologous tissue.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of a medical surgical biological patch. Background technique [0002] Biological patch is a human regeneration system, which can not only repair the host's defective tissue or organ, but also guide the regeneration of endogenous tissue. [0003] In the surgical field of hernia, abdominal wall and meninges, biological patches that are immunogenically removed such as decellularization and degreasing are currently the most characteristic biomaterials in regenerative medicine. The principle of repairing tissue defects is "endogenously induced regeneration". That is to attract and regulate the growth and differentiation of host cells in the scaffold to form new self-tissues to replace the implanted materials so as to complete the repair of defects. Clinically, biological patches have been applied to the repair of various tissues or organs, such as: dura / spinal defect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3687A61L27/3691A61L27/3604A61L27/50A61L2430/40
Inventor 岳崇霞宋磊
Owner 成都奇璞生物科技有限公司
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