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Construction method of patient-derived lymphoma immunodeficient mouse xenograft model and application of construction method

A technology for immunodeficiency mice and construction methods, which can be used in pharmaceutical formulations, preparations for in vivo experiments, animal husbandry, etc., and can solve problems such as low coincidence rate, ineffectiveness, and inability to fully simulate the microenvironment in vivo

Inactive Publication Date: 2020-04-07
顾隽
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to use PDX technology, using immunodeficiency mice as carriers, to implant lymphoma patient tissue cells into mice, and construct a patient-derived lymphoma xenograft model in vivo, thereby solving the current in vitro drug sensitivity test and clinical practice of lymphoma. The problem of low compliance rate of curative effect, especially to solve the problem that the traditional mouse xenograft tumor model cannot fully simulate the in vivo microenvironment, resulting in the ineffectiveness of the screened drug in clinical trials, and at the same time provide an effective reference for the formulation of treatment plans for patients

Method used

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Experimental program
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specific Embodiment approach 1

[0027] Specific embodiment one: the construction method of the patient-derived lymphoma immunodeficiency mouse xenograft model of the present embodiment, it is carried out according to the following steps:

[0028] 1. Obtaining human lymphoma cells: For lymphoma patients, if there is a superficial and palpable tumor mass, the tumor tissue is collected by surgical resection.

[0029] 2. Establishing a method for tumorigenesis of transplanted tumors in mice

[0030] ①The tumor tissue was fully mixed with the monocyte separation medium, the mononuclear cells were separated by centrifugation, sorted by magnetic beads and inoculated into mice.

[0031] ②The tumor tissue was cut into small tissue blocks, and the control variable method was used to investigate the difficulty of tumor formation with different tissue volumes, different tissue block sizes, and different inoculation sites (subcutaneous, subrenal capsule, and abdominal cavity), and the minimum cells required for tumor for...

specific Embodiment approach 2

[0035] Embodiment 2: This embodiment differs from Embodiment 1 in that: the tumor removal method described in Step 1 uses hollow needle puncture. Others are the same as the first embodiment.

specific Embodiment approach 3

[0036] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the mononuclear cell separation liquid in step 2 is Ficoll, and the centrifugation is density gradient centrifugation. Others are the same as the first embodiment.

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Abstract

The invention belongs to the field of medicine and successfully introduces a patient-derived lymphoma immunodeficient mouse xenograft model into lymphoma drug research and development and in-vivo drugsensitive tests on related drugs. Compared with tumor in-vitro drug sensitive tests and traditional mouse xengraft models, the constructed patient-derived lymphoma immunodeficient mouse xenograft model can better research and further elaborate occurrence and development mechanisms of tumor, and meanwhile, a more valuable new animal model is provided for research and development of anticancer drugs. The construction method is applied to the field of construction of mouse models.

Description

technical field [0001] The invention relates to a method for constructing a patient-derived lymphoma immunodeficiency mouse transplanted tumor model and its application, which is used to solve the problem of low coincidence rate between lymphoma in vitro drug sensitivity test and clinical curative effect. Background technique [0002] Tumor is a frequently-occurring and common disease that seriously threatens human health, and its pathogenesis has not been fully elucidated. Lymphoma is a malignant tumor that can be divided into two categories: Hodgkin's Lymphoma (HL) and Non-Hodgkin's Lymphoma (NHL). NHL is the most common, accounting for about 85%. In recent years, the incidence of NHL has been increasing year by year. The incidence rate ranks 6th among male malignant tumors, 7th among female malignant tumors, and ranks 3rd among 0-14 year olds. It is a serious threat to human beings. healthy. [0003] For patients with different types of lymphoma, the treatment strategi...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/0271A01K2207/12A01K2227/105A01K2267/0331A61K49/0008
Inventor 顾隽
Owner 顾隽
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