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Application of oyster peptide in promoting marine shewanella to induce juvenile mytilus coruscus to adhere

A technology of Shewanella and thick-shelled mussels, which is applied in the application field of oyster peptides in promoting the adhesion of marine Shewanella-induced thick-shelled mussels juveniles

Active Publication Date: 2020-04-07
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Oysters are a kind of organisms that are very easy to attach to each other. Whether the oyster peptides prepared from them can also attract the attachment of spat has not been reported in the relevant prior art.

Method used

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  • Application of oyster peptide in promoting marine shewanella to induce juvenile mytilus coruscus to adhere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1 cultivates marine Shewanella

[0013] The pure strains of Shewanella marine were plated and streaked on 2216E solid medium to obtain a single colony, and then transferred to Zobell 2216E liquid medium for expansion, centrifuged and washed to make a suspension. Then stained with acridine orange and randomly selected 10 points under a fluorescent microscope at 1000 times for counting, and determined that the total density of the bacterial liquid was 4.3×10 9 cells / mL.

Embodiment 2

[0014] Example 2 Induction Experiment of Marine Shewanella to Attachment of Thick-shelled Mussel Juveniles

[0015] The initial bacterium liquid density among the selected embodiment 1 is 1 * 10 8 cells / mL marine Shewanella liquid, put a sterilized glass slide in a sterilized petri dish, add a certain amount of sterilized filtered seawater and bacterial liquid containing different concentrations of oyster peptides to make the liquid in the petri dish total The volume is 20mL, the concentration of oyster peptide is 0mg / mL, 0.1mg / mL, 0.3mg / mL, 0.5mg / mL and 1mg / mL, and it is cultured at 18°C ​​in the dark for 48h to form a biofilm. Transfer a glass slide with a biofilm to a new petri dish containing sterilized filtered seawater, and put 10 thick-shelled mussel spat (shell length 1.22±0.05mm, shell height 0.75±0.02mm), record the attachment rate of spat at 24h, that is, the percentage of the number of spat attached to the glass slide to the total number of spat in the petri dis...

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Abstract

The invention discloses application of oyster peptide in promoting marine shewanella to induce juvenile mytilus coruscus to adhere. The marine shewanella is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC No: 9798, the concentration of the oyster peptide is 0.01-1 mg / mL, and the oyster peptide is oyster oligopeptide with the oligopeptide mass percentage content not lower than 65% and the molecular weight smaller than 1 kDa. The oyster peptide has the promoting effect on inducing juvenile mytilus coruscus to adhere through the marine shewanella, aquaculture of mytilus and stability of mytilus populations in the ocean are facilitated, and the oyster peptide has important significance in exploring the juvenile mytilus coruscus adhering mechanism and cultivating and breeding of mytilus coruscus.

Description

technical field [0001] The present invention relates to the application of oyster peptide, in particular to the application of oyster peptide in promoting the attachment of marine Shewanella (Shewanellaloihica) induced mussel spat. Background technique [0002] Thick-shelled mussels (Mytilus coruscus), belonging to the mollusca (Mollusca), bivalve (Bivalvia), mytilodia (Mytilodia), mytilidae (Mytilidae), are distributed in the Yellow Sea, Bohai Sea and East China Sea coasts. Important marine shellfish cultured species. The thick-shelled mussel develops from fertilized eggs to eye-spot larvae, then undergoes attachment metamorphosis, develops into juveniles, and finally develops into adults. During the growth and development process from spat to adult shell, when the environment changes, the thick-shelled mussel spat can cut off the silk by itself, start to crawl and re-select a suitable attachment substrate to attach again. The cultivation and stabilization of mussel popul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54C12N1/20C12R1/01
CPCA01K61/54C12N1/20Y02A40/81
Inventor 梁萧常睿珩彭莉华杨金龙
Owner SHANGHAI OCEAN UNIV
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