A culture medium for promoting porcine oocyte maturation in vitro

Abstract

The invention discloses a culture solution for promoting the in vitro maturation of pig oocytes. The culture solution is based on the M199 culture solution without HEPES, and is also added with porcine follicular fluid, L-cysteine, sodium pyruvate, Epidermal growth factor, insulin, gonadotropins, chorionic gonadotropin, and WNT5A cytokines. Using this culture medium to culture porcine oocytes in vitro can effectively improve the efficiency and quality of in vitro maturation of oocytes, and the mature oocytes thus obtained can be used for the production of pig in vitro fertilization and somatic cell nuclear transfer embryos, and the blastocysts develop Efficiency and quality have also been significantly improved.

Description

Technical field [0001] The present invention belongs to the field of livestock embryo engineering, and more particularly to a culture solution that promotes the body of porcine oocytes. Background technique [0002] In vitro fertilization and body cell nuclear transfer technology is the most common technologies in the field of livestock embryo engineering, which can be applied to the expansion of good livestock, animal model construction, transgenic animal production. However, the embryos obtained by the above techniques have many developmental problems, including low cystic development rate, and less cell group cells, low efficiency of animals. A large reason that causes these problems is that the mood of the oocytes cannot accurately reduce the microenvironment provided by the parent, resulting in a significantly weaker oocyte in vivo in vivo mature oocytes. [0003] At present, the embryonic factor added to the parent secretion in the culture solution can achieve the purpose o...

AI Technical Summary

Problems solved by technology

However, embryos obtained by the above techniques have many developmental problems, including low rate of blastocyst development, low number of cells in the inner cell mass, low birth efficiency of animals, etc.

One of the main reasons for these problems is that the culture system for in vitro maturation of oocytes cannot accurately restore the microenvironment provided by the mother, resulting in significantly weaker developmental potential of in vitro matured oocytes than in vivo matured oocytes.

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