Escherichia coli ghost capable of realizing dual-protein expression and preparation method of escherichia coli ghost capable of realizing dual-protein expression

Abstract

The invention discloses an escherichia coli ghost capable of realizing dual-protein expression. The ghost is prepared from dual-expression bacteriolysis plasmids, the dual-expression bacteriolysis plasmids use pETDuet1 as a carrier, besides, an E gene and staphylococcus aureus nuclease A are inserted, and bacteriolysis plasmids capable of realizing dual-protein expression of the E gene and the staphylococcus aureus nuclease A are realized. According to the ghost disclosed by the invention, gene fragments are respectively inserted in two independent multiple clone sites of the carrier, so thatthe problem that two protein structures in expression mutually influence can be effectively solved, besides, degradation of bacterium DNA is realized, the obtained ghost products are high in safety, dual-protein expression is realized, and the dual-protein expression efficiency is high. The escherichia coli ghost prepared from the pETDuet1-E-SNA bacteriolysis plasmids disclosed by the invention can degrade heredity substances (including drug resistance genes and toxin genes) of the escherichia coli, the use safety of the ghost can be effectively increased, and biological potential safety hazard existing in use of the ghost products can be avoided.

Description

technical field [0001] The invention relates to the technical field of preparation of Escherichia coli slough, more specifically, relates to a double-protein expressed Escherichia coli slough and a preparation method thereof. Background technique [0002] In 1985, Lubitz et al. found that the expression of the cleavage gene E of phage PhiX174 in Gram-negative bacteria could cause the bacteria to form transmembrane channels, and the cytoplasm and organelles escaped from the channels to form bacterial empty shells. Called sloughs or spores. Compared with formalin inactivated bacterial vaccines, the preparation process of sloughs does not damage the antigenic structure of bacterial cells, and its immunogenicity is better preserved, so it can be used as a new type of inactivated vaccine, vaccine carrier or Adjuvant use. At present, most of the relevant reports at home and abroad adopt the expression of E gene alone to prepare slough. However, this method cannot achieve the de...

AI Technical Summary

Problems solved by technology

However, there is a problem that the expression efficiency and structure of the two proteins affect each other

Images