Single cell array chip and screening method for ion channel drugs
An array chip, ion channel technology, applied in the field of ion channel drug screening, can solve the problems of high technical threshold, inaccuracy of FlexStation3, poor reproducibility of results, etc., to reduce false positive rate/false negative rate and improve results. Reproducibility, the effect of reducing sample consumption
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0059] According to the preparation method of the present invention, in step (1), the design drawings of the lower microporous chip and the upper microchannel chip can be drawn by using AutoCAD software. Preferably, the resolution of the high-resolution transparent mask can be 25000dpi or above, so as to ensure the fidelity of the graphics of the present invention.
[0060] According to the preparation method of the present invention, in step (2), the silicon wafer is rinsed with absolute ethanol, boiled with Piranha solution, rinsed with ultrapure water to neutrality, dried and other pretreatments, and then applied to subsequent coating deal with. Described silicon wafer pretreatment method can be: first silicon wafer is rinsed with absolute ethanol, to remove the impurity adhering to silicon wafer surface, blow dry; Then silicon wafer is placed in Piranha solution (concentrated sulfuric acid:hydrogen peroxide=3 : 1) Heating and boiling, the heating time is 10-50min, prefera...
Embodiment 1
[0091] Example 1 - Preparation of single cell array chip
[0092] The structure of the single-cell array chip: the upper surface of the lower microwell chip is provided with a number of cylindrical micro-pits, the diameter of the micro-pit is 25 μm, the depth of the micro-pit is 40 μm, and the distance between the centers of adjacent micro-pit is 80 μm, the micro-pit An array of micro-pits arranged in a linear equidistant manner is formed. The upper microchannel chip is provided with two microfluidic channels, and the two ends of each microfluidic channel are respectively provided with inflow through holes and outflow through holes. The microfluidic channel is located on the lower surface of the upper microchannel chip and communicates with the micropit; the inflow through hole and the outflow throughhole communicate the microfluidic channel with the upper surface of the upper microchannel chip. The cross-section of the microfluidic channel is rectangular, the length of the...
Embodiment 2
[0102] Except that the diameter of the micropit is 28 μm, other parameters are the same as in Example 1.
PUM
Property | Measurement | Unit |
---|---|---|
distance | aaaaa | aaaaa |
depth | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com