Application of microRNA-29a-3p as a target for the prevention and treatment of vascular mimicry in glioma

An application, the technology of LAMB2, which is used in medical preparations with non-active ingredients, medical preparations containing active ingredients, anti-tumor drugs, etc.

Active Publication Date: 2021-08-24
SHANDONG UNIV QILU HOSPITAL
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  • Abstract
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Problems solved by technology

Furthermore, although angiogenesis plays a crucial role in the progression of glioma, none of the above studies implicated this process

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  • Application of microRNA-29a-3p as a target for the prevention and treatment of vascular mimicry in glioma
  • Application of microRNA-29a-3p as a target for the prevention and treatment of vascular mimicry in glioma
  • Application of microRNA-29a-3p as a target for the prevention and treatment of vascular mimicry in glioma

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Embodiment 1

[0060] 1. Materials and methods

[0061] 1.1 TCGA database and clinical specimens

[0062] The related content of TCGA database can be downloaded from Betastasis.com (www.betastasis.com). Data analysis was done by GraphPad Prism. Paraffin-embedded glioma tissues (WHO I-IV) and normal brain tissues were collected from 45 patients who underwent neurosurgery at Qilu Hospital of Shandong University. Normal brain tissue samples (n=5) were collected from patients with severe traumatic brain injury who had undergone partial brain resection.

[0063] 1.2 Immunohistochemistry (IHC)

[0064] Sections were heated, deparaffinized, hydrated and placed in sodium citrate buffer (pH 6.0) for antigen retrieval and endogenous peroxidase activity was blocked with 3% hydrogen peroxide. Slides were blocked with 10% normal goat serum and incubated overnight at 4°C with primary antibody (rabbit anti-CD34 monoclonal antibody, diluted 1:500, ab81289; Abcam; UK). Horseradish-peroxidase-conjugated ...

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Abstract

The invention specifically relates to the application of microRNA-29a-3p as a target for prevention and treatment of glioma vascular mimicry. The study of the present invention shows that miR-29a-3p significantly inhibits the ability of glioma cells to invade, migrate and form vascular mimicry, and at the same time reduces the expression of vascular mimicry-related molecular markers. Furthermore, ROBO1 is a direct target of miR-29a-3p, which mediates the regulatory effect of miR-29a-3p on the formation of vascular mimicry in glioma cells. The study of exosomes overexpressing miR-29a-3p also confirmed that they can inhibit the invasion and migration of gliomas and the formation of vascular mimicry by up-regulating the content of miR-29a-3p in glioma cells. Human mesenchymal stem cell exosomes overexpressing miR-29a-3p can be used to counteract the formation of glioma vascular mimicry, and it is expected to be used as a supplement to anti-vascular therapy to improve the prognosis of glioma patients.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment targets for brain glioma, and in particular relates to the application of microRNA-29a-3p as a prevention and treatment target for vascular mimicry in gliomas. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Gliomas account for 80% of all primary brain tumors and are the most prevalent and deadly central nervous system primary tumors in adults. Angiogenesis is one of the prominent features of glioma, which contributes to the poor prognosis of glioma patients. Avastin, an anti-vascular endothelial growth factor (VEGF) drug, is considered to be an important anti-glioma drug. In...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7105A61K47/46A61P35/00A61P35/04
CPCA61K31/7105A61K47/46A61P35/00A61P35/04
Inventor 李刚薛皓郭兴张宗璞徐建业赵荣荣薛付忠刘宏
Owner SHANDONG UNIV QILU HOSPITAL
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