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Extraction method of total saponins of trillium tschonoskii and pharmaceutical application thereof

An extraction method and technology of trillium, applied in the direction of medical preparations containing active ingredients, drug combinations, antineoplastic drugs, etc., can solve the problems of multidrug resistance clinical application, limitations, side effects of chemotherapy drugs, etc.

Inactive Publication Date: 2020-03-31
CHINA THREE GORGES UNIV
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Problems solved by technology

Chemotherapy is still one of the main methods for the treatment of colon cancer, but the serious side effects and multidrug resistance of chemotherapy drugs have greatly limited its clinical application

Method used

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  • Extraction method of total saponins of trillium tschonoskii and pharmaceutical application thereof
  • Extraction method of total saponins of trillium tschonoskii and pharmaceutical application thereof
  • Extraction method of total saponins of trillium tschonoskii and pharmaceutical application thereof

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Embodiment 1

[0022] Drugs and reagents The traditional Chinese medicine Trillium used in the present invention is picked in Shennongjia forest area, and is identified as Trillium by the third-level laboratory of Chinese medicine pharmacology of Three Gorges University. The human colon cancer cell line CT26 was provided by Shanghai Kelei Biotechnology Company. 1640 medium, trypsin and PBS were purchased from Hyclone Company, fetal bovine serum was purchased from Wuhan Saipu Nuo Biotechnology Co., Ltd., dimethyl sulfoxide (DMSO) and tetramethylazolium salt (MTT) were purchased from Sigma Company; β-actin antibody, Ras antibody, phosphorylated Ras antibody, Akt antibody, phosphorylated Akt antibody, Erk antibody, and phosphorylated Erk antibody were purchased from CST Corporation in the United States. Organic solvents such as methanol, petroleum ether, ethyl acetate, and n-butanol were purchased from Hubei Zechuan Technology Co., Ltd.

[0023] Instruments used in the present invention includ...

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Abstract

The invention provides an extraction method of total saponins of trillium tschonoskii and a pharmaceutical application thereof. The result shows that the total trillium saponin can effectively inhibitthe proliferation of colon cancer CT26 cells, and the IC50 of the total trillium saponin is 9.28 mu g / mL. Western blotting results show that the total saponins of the trillium tschonoskii can reducethe expression of Ras, Akt, Erk proteins and phosphorylated proteins thereof in a concentration-dependent manner. In-vivo experiments show that compared with a model group, the total saponins of the trillium tschonoskii have good inhibition rates, namely 51.22%, 34.88% and 25.02% respectively, and the total saponins of the trillium tschonoskii have a certain concentration dependence trend. The HEstaining shows that total saponins of trillium tschonoskii can promote apoptosis of colon cancer cells, and large tumor cell necrosis can be seen in the middle and high-dose groups. The Western blotting results show that the total saponins of the trillium tschonoskii can reduce the expression level of Rss protein in the concentration-dependent manner. The the total saponins of the trillium tschonoskii can effectively inhibit the proliferation of colon cancer CT26 cells, and the mechanism of the the total saponins of the trillium tschonoskii is that the expression of Ras, Akt and Erk proteins is reduced through a Ras signal path.

Description

technical field [0001] The invention relates to a method for extracting trillium total saponins, in particular to the application of a trillium total saponins as a medicine for treating colon cancer. It belongs to the field of biopharmaceuticals. Background technique [0002] Colon cancer is one of the most common malignant tumors in the digestive system, which seriously threatens human health. Chemotherapy is still one of the main means of treating colon cancer, but the serious side effects and multidrug resistance of chemotherapy drugs have greatly restricted its clinical application. Therefore, looking for drugs that can efficiently kill tumor cells while having less toxic and side effects is a hot spot in the research and development of new anti-tumor drugs. [0003] Trillium tschnoskii Maxim is one of the four famous Tujia medicines. It is sweet in taste and warm in nature. Weakness etc. Studies have shown that trillium extracts and saponins have significant inhibit...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61P35/00
CPCA61K36/896A61K2236/15A61K2236/33A61K2236/51A61K2236/55A61P35/00
Inventor 喻玲玲邹坤陈静文陈先勇蒋安蓉李世刚柳蔚
Owner CHINA THREE GORGES UNIV
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