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miRNA for regulating and controlling insect wing development and application of miRNA

An insect and wing-shaped technology, applied in the field of agricultural biology, can solve the problems of increasing the amount of chemical pesticides, environmental pollution, and destroying the agricultural ecological balance, and achieves the reduction of long-winged migratory individuals, regulation of wing-shaped development, and regulation of migration. Effect

Inactive Publication Date: 2020-03-27
ZHEJIANG UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the continuous long-term and large-scale use of chemical pesticides, brown planthoppers gradually show different degrees of resistance to various pesticides, further increasing the amount of chemical pesticides will cause serious environmental pollution, threaten human health and destroy agricultural ecological balance.

Method used

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  • miRNA for regulating and controlling insect wing development and application of miRNA
  • miRNA for regulating and controlling insect wing development and application of miRNA
  • miRNA for regulating and controlling insect wing development and application of miRNA

Examples

Experimental program
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Effect test

Embodiment 1

[0020] (1) According to the mature nlu-miR-34 sequence, Guangzhou Ruibo Biotechnology Co., Ltd. synthesized agomiR-34, an analogue of nlu-miR-34, and synthesized an agomiR-NC control using randomly scrambled nucleic acid sequences as templates.

[0021] (2) Dilute the agomiR-34 and agomiR-NC controls to 2.67ng / nL with nuclease-free water.

[0022] (3) The nymphs of N. lugens on the first day of the third instar were selected, and 15nL of agomiR-34 solution was injected through a glass electrode into the outer epidermis of the mesothorax of N. lugens (treatment group) using a microinjector Nanoinjector. The same amount of agomiR-NC solution was injected into the N. lugens of the control group.

[0023] (4) Use a brush with a soft tip to transfer the injected nymphs to a glass jar filled with fresh rice seedlings, cover tightly with breathable gauze, and tie a rubber band, observe and record the death of brown planthoppers every day, and continue to raise them until Feathering....

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Abstract

The invention provides a miRNA for regulating and controlling insect wing development and an application of the miRNA. The miRNA bioinformatics is used to obtain the nilaparvata lugens specific miRNA:nlu-miR-34, in-vitro synthetic nlu-miR-34 analogue agomiR-34 is injected into nilaparvata lugens, and results show that the analogue agomiR-34 has a lethal effect on the nilaparvata lugens, and can significantly regulate and control the wing development of the nilaparvata lugens.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and specifically relates to a miRNA for regulating insect wing shape development and an application thereof. Background technique [0002] The brown planthopper, Nilaparvata lugens, belongs to Hemiptera and belongs to the planthopper family. It is one of the most serious frequent pests of rice in my country. The brown planthopper has migratory habits. It migrates to my country from Southeast Asia every spring under the influence of the monsoon, and can migrate back in autumn. It is currently the primary pest of rice in my country, Japan, Thailand, Vietnam, Indonesia and many other Asian countries. The brown planthopper has a strong ability to adapt to different environmental conditions, which is inseparable from the phenomenon of the second type of wing-the long-winged type can escape from unfavorable environments by flying and find new food sources; the short-winged type is not good at ...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/89C12N15/11
CPCC12N9/1007C12N15/11C12N15/1137C12N15/89C12N2310/10C12N2310/14C12Y201/01037
Inventor 徐乐汤沈杨贺康李飞
Owner ZHEJIANG UNIV
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