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Application method of antibacterial peptide in rainbow trout breeding period

An application method and technology of rainbow trout, applied in the field of biological preparations, can solve problems such as bacterial resistance, affect the quality of aquatic products, and endanger human health, so as to increase the expression level, enhance the body's disease resistance, and improve the survival of seedlings rate effect

Pending Publication Date: 2020-03-27
FISHERIES SCI RES INST OF JILIN PROVINCE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, antibiotics used as veterinary drugs and feed additives are widely used, which destroys the micro-ecological balance of the aquaculture water environment, causes drug residues in fish, and causes bacterial resistance, which affects the quality of aquatic products and endangers human beings. health

Method used

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  • Application method of antibacterial peptide in rainbow trout breeding period
  • Application method of antibacterial peptide in rainbow trout breeding period
  • Application method of antibacterial peptide in rainbow trout breeding period

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Experimental program
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Effect test

Embodiment Construction

[0016] A method for applying antimicrobial peptides in the breeding period of rainbow trout is characterized in that: soaking eye eggs of rainbow trout with antimicrobial peptides in the breeding period of rainbow trout, including the following steps, and the following steps are carried out in sequence,

[0017] Step 1, using an antimicrobial peptide with an active unit of 210U / ml, adding 4 times the volume of water for dilution to obtain an antimicrobial peptide soaking solution;

[0018] Step 2. Add the antimicrobial peptide soaking liquid obtained in Step 1 into the rainbow trout egg container, and soak for 3 minutes. During the soaking period, the dissolved oxygen in the container is 7mg / L-9mg / L;

[0019] Step 3. According to the operation method of step 2, soak the eye-eyed eggs of rainbow trout once every other day, and after soaking for 10 times, carry out the next stage of cultivation of rainbow trout.

[0020] The specific group test conditions are as follows:

[002...

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PUM

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Abstract

The invention discloses an application method of antibacterial peptide in a rainbow trout breeding period, and belongs to the technical field of application of biological agents. The healthy antibacterial and antiviral preparation, namely the antibacterial peptide, is applied to the rainbow trout breeding period under the conditions of the most proper dilution ratio, soaking time, soaking frequency and soaking times, and eyed eggs of rainbow trout in the breeding period are soaked. The expression quantity of immune-related enzymes and cell factors of the rainbow trout can be remarkably increased, the body disease resistance of the rainbow trout in the breeding period is enhanced, the fry survival rate of the rainbow trout in the breeding period is effectively increased, and the applicationmethod is an important direction for prevention and control of epidemic diseases of aquaculture animals in future and has important significance in water environment safety, aquatic product quality safety and human health.

Description

technical field [0001] The invention belongs to the technical field of application of biological preparations, in particular to an application method of antimicrobial peptides in the breeding period of rainbow trout. Background technique [0002] Antimicrobial peptide is a kind of immune enhancer with broad-spectrum antibacterial effect. When the body encounters pathogen invasion and environmental stress, it can exert its immune regulation function and activate the immune system of fish. Initiate and regulate the host immune defense system, exert immune function, and play a significant role in the biological defense system. At the same time, as a new type of polypeptide drug, it is a kind of membrane active polypeptide with broad-spectrum bactericidal, anti-fungal, anti-virus, anti-tumor cell and other functions. In recent years, it has attracted much attention among many immune enhancers, and has become a research hotspot in many fields such as animal husbandry, medicine,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/13
CPCA01K61/13Y02A40/81
Inventor 闫春梅郑伟韩叶李忠强肖志国王秀兰马驰王战蔚刘慧吉
Owner FISHERIES SCI RES INST OF JILIN PROVINCE
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