HPLC quantitative analysis method for aesculin and aesculetin in pulsatilla chinensis powder

Abstract

The invention discloses an HPLC quantitative analysis method for aesculin and aesculetin in pulsatilla chinensis powder. The HPLC quantitative analysis method specifically comprises the following steps: step 1, determining chromatographic conditions; step 2, extraction process: precisely weighing pulsatilla chinensis powder, putting the pulsatilla chinensis powder into a conical flask with a plug,precisely adding methanol and a sealing plug, weighing, carrying out ultrasonic extraction, cooling, weighing again, supplementing the reduced weight with methanol, uniformly shaking, filtering, andtaking the subsequent filtrate; step 3, preparing a solution: preparing a reference substance storage solution, a reference substance solution, a test solution, a negative reference solution and a reference medicinal material solution; step 4, methodological investigation, including specificity test, linear relationship investigation, precision test and accuracy test; and step 5, sample determination: determining the content of a self-made pulsatilla chinensis powder sample and determining the content of commercially available pulsatilla chinensis powder. The method provided by the invention has the technical effect that the effective components aesculin and aesculetin in the pulsatilla chinensis powder can be accurately quantified.

Description

technical field [0001] The invention relates to the technical field of content determination of drug index components, in particular to an HPLC quantitative analysis method for teretine and terperetin in pulsatilla powder. Background technique [0002] Pulsatilla powder, which is recorded in the second part of the 2015 edition of the "People's Republic of China Veterinary Drug Code", is a classic prescription of Chinese veterinary medicine powder, which is composed of Pulsatilla, Coptis chinensis, Phellodendron Phellodendron and Qinpi. Among them, Pulsatilla grandis is the monarch drug, which has the functions of clearing heat and detoxifying, cooling blood and stopping dysentery; Qinpi, Cortex Phellodendri, and Coptis chinensis are adjuvant drugs for clearing heat and detoxifying, drying dampness and stopping dysentery. The 4 medicinal materials are used together to clear heat and detoxify, cool blood and stop dysentery. Indications: heat poison red dysentery, damp heat dy...

AI Technical Summary

Problems solved by technology

[0003] However, the traditional character identification and microscopic identification methods are often unable to distinguish this similarity, resulting in many problems in the use of the preparation, such as insufficient addition of medicinal materials, shoddy, etc., and even commercially available pulsatilla powder The main components of cerebri and cerebri were not detected, etc.

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